The Metabolomics Core Facility provides services for the analysis of metabolites and lipids.

The facility is equipped with a high-resolution mass spectrometry system (HRMS)triple quadrupole with an ion trap (QTRAP) mass spectrometer coupled with ultra-high performance liquid chromatography (UHPLC),  and direct infusion system (NanoMate) for separation and detection of various classes of small molecules, metabolites, lipids, and drugs.

Major projects and accomplishments

  • Identification and detection of various classes of small molecules, metabolites, and lipids in mammalian and microbial cells

  • EMBL-MCF mass spectral library from over 700 metabolites

  • Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, free fatty acids, etc)

  • Developed protocols to analyze over 100 metabolites in a targeted manner

  • Relative quantitation of metabolites and lipids using internal standards

  • Analysis of drugs and related metabolites

Services provided

We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids. We perform targeted and untargeted measurements of various classes of molecules ranging from small metabolites to lipids in a variety of biological matrices.

Further services

  • Global untargeted metabolomics and lipidomics profiling
  • Targeted metabolomics profiling
  • Free fatty acid analysis
  • Metabolite and lipid identification using MS and MS/MS analysis

  • Drug and drug metabolite analysis
  • Low-volume high-throughput direct infusion analysis (with Advion Nanomate)

Acknowledgment and Authorship:

As per EMBL Internal Publication Policy (No. 63); if the facility services were used in  work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:

“We acknowledge the support of the EMBL Metabolomics Core Facility  (MCF)  in the acquisition and analysis of mass spectrometry data”

In addition, If a staff member of the Metabolomics Core Facility contributes substantially to published work, the staff member should be included as an author in the published work.

Services