The Metabolomics Core Facility provides services for the analysis of metabolites and lipids.
The facility is equipped with a high-resolution mass spectrometry system (HRMS), triple quadrupole with an ion trap (QTRAP) mass spectrometer coupled with ultra-high performance liquid chromatography (UHPLC), and direct infusion system (NanoMate) for separation and detection of various classes of small molecules, metabolites, lipids, and drugs.
Major projects and accomplishments
Identification and detection of various classes of small molecules, metabolites, and lipids in mammalian and microbial cells
EMBL-MCF mass spectral library from over 700 metabolites
Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, free fatty acids, etc)
Developed protocols to analyze over 100 metabolites in a targeted manner
Relative quantitation of metabolites and lipids using internal standards
Analysis of drugs and related metabolites
We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids. We perform targeted and untargeted measurements of various classes of molecules ranging from small metabolites to lipids in a variety of biological matrices.
- Global untargeted metabolomics and lipidomics profiling
- Targeted metabolomics profiling
- Free fatty acid analysis
- Metabolite and lipid identification using MS and MS/MS analysis
- Drug and drug metabolite analysis
- Low-volume high-throughput direct infusion analysis (with Advion Nanomate)
Acknowledgment and Authorship:
As per EMBL Internal Publication Policy (No. 63); if the facility services were used in work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:
“We acknowledge the support of the EMBL Metabolomics Core Facility (MCF) in the acquisition and analysis of mass spectrometry data”
In addition, If a staff member of the Metabolomics Core Facility contributes substantially to published work, the staff member should be included as an author in the published work.