SDS-PAGE analysis of His-TEV purification by ion exchange.

The Protein Expression and Purification Core Facility produces and purifies proteins both from Escherichia coli and from insect cells. We implement a large array of chromatographic techniques and also provide support for the biophysical characterisation of the purified proteins.

The main aim of the facility is to provide assistance and scientific advice concerning all experiments related to protein expression, purification and biophysical characterisation. We can perform quality control assays probing the folding state and stability of the recombinant proteins. We strive to stay up-to-date with the latest scientific developments and trends in the field and test out new products and protocols for our users. Furthermore, we maintain an extensive collection of expression vectors and bacterial strains and we also have some frequently used proteins such as proteases and polymerases readily available. This helps to considerably reduce the expenses of our users.

Services provided

  • Expression testing of the protein of interest in various E. coli and insect cell strains and in different culturing conditions.
  • Purification of recombinant proteins from E. coli and insect cells.
  • Quality control and biophysical characterisation of purified proteins (for example: analytical gel filtration, analytical ultracentrifugation, ITC, CD, and thermofluor assays).
  • Preparation of injection material for immunisations and purifications of antibodies from serum.
  • Production of frequently used proteins (for example: proteases, polymerases and fluorophores) for general use within EMBL.
  • Providing scientific and technical advice to internal and external researchers.
  • Teaching and training of users in all aspects of protein expression and purification techniques.
  • Maintaining a large collection of expression vectors and bacterial strains.
  • Evaluating new protocols and products for our users.

Technology partners

We are open to collaborations with academic and industrial partners to assess new products or technological developments. Furthermore, we have initiated a network of protein facilities across Europe called Protein Production and Purification Partnership in Europe (P4EU) to improve information exchange and the evaluation of novel technologies.