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This course is aimed at Technicians and Scientists interested in microinjection into adherent cells. Participants should have basic experience with cell culture and microscopy.
20% of the course programme covers lectures and tutorials on microinjection and corresponding microscopy techniques by experts in the field. 80% focuses on hands on practical work organised in small groups. These practicals will address the following topics: sample preparation, microinjection of marker dyes, microinjection of GFP-constructs, microscopy training, and experiment analysis.
Participants may bring along their own samples as they will have the possibility to image them and discuss results with the course teachers.
This course is co-organised with Eppendorf