Location & dates EMBL Heidelberg, Germany 26 - 28 Feb 2013
Deadlines Application closed

(Preliminary) Programme

Day 1 – Tuesday, 26 February 2013
09:00 Welcome
  Intro to Transgenics
  Overview of ZFNs and Gene Modification
  Pronuclear Injection
  Lab Practical: Microinjection
  Creating Constitutive Knockout Models
  Lab Practical: Lab Overview, Deletion and Mismatch PCR Setup
17:00 Gene Insertion and Conditional Knockout
17:30 Dinner at EMBL



Day 2 - Wednesday, 27 February 2013
09:00 Lab Practical: Load deletion PCR gels; Set up Mismatch Formation
  Considerations for Cell-based Transgenics
  Donor Design
  ZFN Design
  Lab Practical: Mismatch Detection Assay & PAGE & Troubleshooting
  Talen Technology: What's the difference?
17:00 Lab Practical: Set up Junction & RFLP PCR
19:00 Dinner downtown



Day 3 - Thursday, 28 February 2013
09:00 Lab Practical: Load jPCR gel; Cleanup RFLP & Digest
  In vivo Therapeutic Applications of ZFN
  SAGE Labs Transgenic Model Creation Service
  Group Photo
  Lab Practical: Run RFLP gel
  Guest Lecture
  Exercises for ZFN Application
  Experimental Results and Analysis
16:00 Closeout