The pre-conference workshop will be hosted by Singer Instruments during registration for the EMBO Conference "Experimental Approaches to Evolution and Ecology using Yeast and Other Model Systems".
Date: Sunday, 12 October 2014
Time: 16:30 - 17:30
Venue: EMBL Advanced Training Centre, Courtyard Room A
Participation in the workshops is first come - first served.
The recent advances in robotic automation for genome-wide high-throughput genetic screens in yeast and other microbial organisms
Jay Yang, Singer Instruments
Yeast is an ideal model organism for high-throughput genetic screens because of its experimental tractability and the availability of Yeast Knockout Collections and other powerful genetic tools. Methodologies such as synthetic genetic array analysis (SGA), yeast two-hybrid screening (Y2H), haploid insufficiency profiling / homozygous deletion profiling (HIP/HOP), and multicopy suppression profiling (MSP) have been developed for the systematic and comprehensive mapping of genetic and physical interactions, allowing the researchers to assess the gene functions, delineate complex genetic networks and identify drug-target interactions in an unbiased manner. As these methodologies mature and become popular, there is an increased demand for having reliable and easy-to-use platforms to increase screening throughput and alleviate human errors.
Here, I will present three recent developments in robotic automation for high-throughput genetic screens: 1) an ultra-high-density, 6144 colony arraying system, 2) a turnkey solution for plate imaging, documenting and analysis, and 3) a compact automatic plate-pouring device. I will then discuss how these new developments could advance the field of high-throughput genetic screens in yeast and other microbes, making it more accessible to the general research community.