Location & dates EMBL Heidelberg, Germany 8 - 10 Oct 2014
Deadlines Application closed Abstract submission closed

Programme

Day 1 - Wednesday 08 October 2014
TimeTopic
09:30 - 10:45 Session 1 - Seminar
Welcome
Introduction to Illumina Sequencing & sample prep
10:45 - 11:45 Session 2 - Laboratory
RNA quantification
11:45 - 12:30 Lunch
12:30 - 15:15 Session 3 - Laboratory
RiboZero depletion of RNA
RNA XP bead clean up
Fragment RNA
Set up reverse transcription
15:15 - 16:00 Run reverse transcription  /  Coffee break
16:00 - 17:30 Session 3 continued - Laboratory
Add 2nd strand mix
2nd strand synthesis  /  Sequencing controls
Clean up of double stranded cDNA
17:30 - 17:45 Coffee break
17:45 - 18:00 Clean up of double stranded cDNA
18:00 - 19:00 Dinner in cafeteria

 

Day 2 - Thursday 09 October 2014
TimeTopic
08:30 - 08:45 Session 4 - Seminar
Quick review and day plan
08:45 - 10:30 Session 5 - Laboratory
Set up adenylation
Run adenylation  /  Troubleshooting and best practices
Set up ligation
10:30 - 10:45 Run ligation  /  Coffee break
10:45 - 12:15 Session 5 continued - Laboratory
Bead wash 1
12:15 - 13:15 Lunch
13:15 - 14:45 Session 5 continued - Laboratory
Bead wash 2
14:45 - 15:15 Session 6 - Laboratory
Set up PCR
15:15 - 15:30 Coffee break
15:30 - 18:00 Session 6 continued - Laboratory
Run PCR  /   RNA Sequencing
                     Jonathon Blake,
                     EMBL Heidelberg, Germany
Clean up PCR
18:00 - 19:00 Dinner in EMBL cafeteria

 

Day 3 - Friday 10 October 2014

Time

Topic

09:00 - 10:00 Session 7 - Seminar
Library preparation review
Library validation
10:00 - 10:30 Coffee break
10:30 - 12:30 Session 8 - Laboratory
Set up qPCR for library quantification
Set up Fragment Analyzer for Library Valildation
12:30 - 13:30 Lunch
13:30 - 16:30 Session 9 - Seminar
Review of library validation results
RNA Seq data analysis using BaseSpace Apps
Course feedback and wrap up