Location & dates EMBL Heidelberg, Germany 15 - 23 Mar 2015
Deadlines Application closed

Practical content

Tom Surrey:
A biochemical in vitro assays will be set up for the study of purified microtubule binding proteins. Using TIRF microscopy we will image individual binding/unbinding events over time. Dwell time and waiting time distributions will be extracted from the recorded movies, using Image J and Matlab, and will then be used to calculate (concentration-dependent) dissociation constants.

Ana Garcia-Saez:
In this practical we will learn state of the art FCS based technologies.

Celine Maeder:
In this practical we will learn state of the art SPIM based technologies.

Sigrid Milles:
Flexible protein systems that take up multiple conformations require analysis techniques that can distinguish, and handle dynamic shuttling between and within different states. We will employ state of the art fluorescence spectroscopy to analyse labeled proteins on a molecule by molecule basis. Exploiting various photophysical properties, such as fluorescence intensity, lifetime and polarization across a wide range of time scales down to picoseconds will allow us to interrogate state-specific protein plasticity at high temporal resolution.

Marina Kuimova:
In this practical we will learn state how to sense rotational motions using fluorescence.

Stefan Hell:
In this practical we will learn state of the art STED based technologies.

Michael Knop:
In this practical we will use state of the art FCS/FCCS instruments and a new SPIM for the detection and visualisation of fast protein mobilities inside cells.

Edward Lemke:
To extract as much information as possible about biomolecular motion, single molecule studies benefit from long-term observation to collect as many photons as possible.  In this practical, we will use particle tracking assays to achieve this either in the context of state of the art multiplexed microfluidic technologies, or so called rconstituted transport assays.

Malte Wachsmuth:
In this practical we will learn state of the art FCS/FCCS based technologies.

Jonas Ries:
This practical course will provide hands-on experience on different modalities of localization microscopy including 3D and multi-color on mammalian and yeast cells using several different labeling strategies. We will learn how to prepare the samples, how to take data on a home-built setup and how to analyze it.

Lars Hufnagel:
In this practical we will learn state of the art SPIM based technologies.

Ilka Bischoffs:
Fluorescence timelapse microscopy is a powerful tool for the quantitative analysis of diverse dynamic processes in bacterial systems.  The course covers the individual steps that are required to successfully conduct such an experiment: starting from the design of appropriate fluorescence reporter constructs, the set-up automated imaging experiments to the quantitative analysis of the data by image processing. The theory and principles of the individual techniques will be introduced and trained through hands-on sessions.