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This course will teach participants the practical aspects of live-imaging plant tissues. Plants are quite different to animals in many ways. For instance they typically develop much more slowly and cells do not migrate. Their overall morphologies are also very different and the presence of cell walls presents challenges optically. Over many years, certain laboratories have developed specialised imaging techniques optimised for plants. However despite these advances, the adoption of these techniques by the wider plant research community has been extremely limited. These specialised techniques will be taught to help promote their wider adoption. The course will cover the physical preparation of samples from different parts of the plant as well as time-lapse imaging strategies and image analysis approaches and uses.
The main practical themes will include:
- Confocal imaging of developing shoot meristems and leaves
- Light-sheet imaging of roots
- In vivo imaging of calcium and pH
- Kinetic microscopy techniques, such as FRAP and FLIM-FRET.
Lectures on many other related topics such as image segmentation, modeling and Atomic Force Microscopy (AFM) will also be presented.