Registration is no longer possible for this course. The course is fully booked.
Why You Should Apply
This course will present flow cytometry and laser microdissection as methods to obtain single cells for subsequent gene expression analysis. We will present 3 different techniques to assess gene expression: qRTPCR, Next Generation Sequencing and the Fluidigm system, as well as methods for single-cell protein quantification. The collected data will be analysed in a comprehensive session.
The participants will learn about the complete workflow and gain understanding about the possibilities and limitations of the approach. The course will cover lectures and hands-on sessions by experts in the fields and is aimed for PhD students, postdoctoral fellows or other scientists with a solid qPCR and molecular biology background.