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Genomic and proteomic approaches have enabled breakthrough advances in the understanding of chromatin biology. This course attempts to integrate these omics approaches to understand the principle of chromatin operation and its biological relevance. Using a murine embryonic stem cells as a tractable model system, this course aims to analyze the composition and variation of chromatin components, and the “epigenetic landscape” at key time points following the induction of cellular differentiation.
This course is aimed at PhD students and post-doctoral researchers who seek to assess chromatin reorganization and/or are working in the field of neuronal development.
- Culture and neuronal differentiation of murine stem cells
- Chromatin sample preparation for genomic and proteomic analysis
- Meta-analysis of post sequencing data
- Integration of MS proteomic data
After this course participants should be able to:
- Cultivate and differentiate neuronal murine stem cells
- Prepare chromatin samples for genomic and proteomic analysis
- Integrate genomic and proteomic data