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High-throughput techniques such as protein analysis by mass spectrometry, or high-throughput genome-scale DNA, RNA sequencing are successfully applied to diverse biological questions with the goal of reaching a comprehensive description of their molecular regulation.
However, they cannot provide temporal or spatial resolution nor directly show that the identified molecules have a function in the dynamic cellular process investigated. Quantitative fluorescence-microscopy in living cells overcomes these limitations because it can probe the function of macromolecules in living cells with ever increasing spatial and temporal resolution.
Imaging-based assays enable genome-wide functional analyses by high-throughput microscopy if combined with standardized reagents that systematically interfere with gene expression or protein activity (e.g. siRNAs, sgRNAs in conjunction with CRISPR/Cas9, chemical inhibitor libraries). In addition, new GFP-tagging genome-editing-based approaches now also allow systematic direct characterization of the abundance, localization, dynamics and interaction of proteins in intact cells, and therefore hold the promise of imaging-based proteomics in single living cells.
High-throughput microscopy techniques that can provide the data for such systems-biology questions require a high level of automation, quantification and data integration and mining and therefore precise coordination of various steps in a large scale workflow.
The course is intended for PhD as well as post-doctoral students from universities and research institutes. Staff of the host institute will not participate. One or two outstanding applicants from industry may be considered but participation will be at their own costs. Selection will be on the basis of the following criteria:
a) Need for immediate application of the taught techniques to their own projects.
b) Evaluation of their competence based on the motivation for their application and their CV.
All other criteria will be based on the usual EMBO policy.
The course is an excellent means to fill the need of European scientists to become acquainted with novel and cutting-edge developments on High-Throughput Microscopy, which is becoming a key technology for systems biology.