Location & dates EMBL Heidelberg, Germany 17 - 23 Apr 2016
Deadlines Application closed

Final Programme

Day 1 - Sunday 17 April 2016
TimeSpeaker
14:00-14:30 Registration and Welcome
14:30-15:00 Welcome address, Introduction, Overview of the course
15:00-16:00

Introduction to the CLEM, historical overview

Correlative Microscopy: 1 + 1 = 3
Paul Verkade, University of Bristol, UK

16:00-16:20

Coffee Break / Discussion

16:20-18:45

Flash talks / Meet the participants

19:00 Dinner
Day 2 - Monday 18 April 2016
TimeSpeaker
9:00-10:00

Experimental setup for high-precision room-temperature CLEM: From sample preparation to data acquisition
Wanda Kukulski, MRC Laboratory of Molecular Biology, UK

10:00-10:30

Coffee Break

10:30-11:30

Bridging the gap: linking structure to function with integrated microscopy
Christopher Peddie, The Francis Crick Institute, UK

11:30-12:30

Introduction to Cryo CLEM
Martin Schorb, EMBL Heidelberg, Germany

12:30-13:15

Lunch

13:15-13:30

Safety information for the practicals
Yvonne Yeboah, EMBL Heidelberg, Germany

13:30-14:15

Introduction to Practicals: Getting to know the basics
Rachel Mellwig, EMBL Heidelberg, Germany
        Sample preparation for Plastic CLEM
        Light microscopy for Plastic sections
        Electron Microscopy
        Sample preparation for Cryo
        Light microscopy for Cryo sections

14:30-16:30

Basic Practical Sessions 1

16:45-18:45

Basic Practical Sessions 2

19:00

Dinner kindly sponsored by Leica Microsystems

Day 3 - Tuesday 19 April 2016
TimeSpeaker
9:00-10:00

Exploring the third dimension with correlative microscopy
Christopher Peddie, The Francis Crick Institute, UK

10:00-10:30

Coffee Break / Discussion

10:30-12:30

Basic Practical Sessions 3

12:30-13:30

Lunch

13:30

Group picture

13:30-15:30

Basic Practical Sessions 4

15:30-17:30

Basic Practical Sessions 5

17:45-18:45 CLEM: Applications for biology
Paul Verkade
, University of Bristol, UK
19:00

Dinner downtown

Day 4 - Wednesday 20 April 2016
TimeSpeaker
9:00-10:00

Using a hybrid imaging approach to dissect the mechanisms of endocytic membrane remodelling
Wanda Kukulski, MRC Laboratory of Molecular Biology, UK

10:00-11:00

Multiscale and multidimensional Image registration for CLEM : techniques and pitfalls
Perrine Paul-Gilloteaux, University of Nantes, France

11:00-11:30

Coffee Break / Discussion kindly sponsored by Science Services

11:30-12:30

Introduction to Computer Practicals
Martin Schorb, EMBL Heidelberg, Germany
Perrine Paul-Gilloteaux, University of Nantes, France

12:30-13:30

Lunch

13:30-17:30

Computer Practicals

17:30-18:15

Introduction to Advanced Practicals

19:00

Dinner

Day 5 - Thursday 21 April 2016
TimeSpeaker
9:30-10:30 Cryo-CLEM: a user's review
Ori Avinoam, EMBL Heidelberg, Germany
10:30-11:00

Coffee Break / Discussion

11:00-12:00

Towards a workflow for cryo-TEM preparation in structural biology
Alexander Rigort, FEI, The Netherlands

12:00-12:30

Tricks and pitfalls in ultramicrotomy
Helmut Gnaegi, Diatome, Switzerland

12:30-13:30

Lunch

13:30-15:45

Advanced Practicals 1

Free afternoon

Day 6 - Friday 22 April 2016
TimeSpeaker
9:00-10:00

EM sample preparation solutions
Thorben Kurz, Leica, Austria

10:00-12:15

Advanced Practicals 2

12:30-13:30

Lunch

13:30-15:45

Advanced Practicals 3

16:00-18:15

Advanced Practicals 4

18:30

Dinner in the Canteen

Day 7 - Saturday 23 April 2016
TimeSpeaker
9:00-9:45 CLEM from the perspective of a core facility
Yannick Schwab, EMBL Heidelberg, Germany
9:45-10:30 Not only yeast: tips and tricks to adapt on-section CLEM to diverse biological questions
Paolo Ronchi, EMBL Heidelberg, Germany
10:30-10:45 Coffee Break
10:45-11:30 Monitoring nuclear envelope and nuclear pore assembly by CLEM
Shotaro Otsuka, EMBL Heidelberg, Germany
11:30-12:30 Concluding remarks / feedback from participants
12:30-13:30 Lunch and Farewell