Location & dates EMBL Heidelberg, Germany 13 - 16 Mar 2016
Deadlines Registration closed Abstract submission closed
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Conference Programme

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Day 1 - Sunday 13 March 2016
TimeSpeaker
14:00-15:30 Registration
15:30-16:00 Welcome remarks

Jan Ellenberg, EMBL Heidelberg

Markus Weber, CEO of Carl Zeiss Microscopy GmbH, Germany
16:00-17:00

Keynote Lecture 1
Structural Neurobiology: goals, tools, pitfalls
Winfried Denk, Max Planck Institute of Neurobiology (MPIN), Germany

17:15-18:15

Keynote Lecture 2
Machine Learning in automated EM volume tracing
Fred Hamprecht, IWR, Heidelberg University, Germany

18:15-18:30

Housekeeping announcement

18:30-19:30

Welcome reception

19:45

Bus to ISG and downtown

Day 2 - Monday 14 March 2016
TimeSpeaker
Session 1
3D Correlative Light and Electron Microscopy
Chair: Graham Knott, EPFL Lausanne, Switzerland
09:00-09:30 Registration
09:30-10:15 Analysing neuronal plasticity using focussed ion beam scanning electron microscopy
Graham Knott, EPFL Lausanne, Switzerland
10:15-10:30 No two CLEM experiments are the same: a diversity of challenges
Jemima Burden, MRC Laboratory for Moleular Cell Biology, United Kingdom
10:30-11:15

Coffee Break and Poster Session

11:15-11:45

A 3D CLEM workflow for cultured cells
Lucy Collinson, Francis Crick Institute,  United Kingdom

11:45-12:15

Caught in the Act: High-Resolution 3D Imaging of Single Tumor Cells Invading Mouse Tissue
Matthia Karreman, EMBL Heidelberg, Germany

12:15-12:30

Advancing array tomography for mapping of 3D subcellular localization in C. Elegans
Sebastian Markert, University of Würzburg Germany

12:30-13:30

Lunch and Poster Session
Odd Numbers

13:30-15:00 Workshops

FIB-SEM (Location: EMCF room 309)
Sample Preparation (Location: EMCF room 305A)
3View (Location: ATC Flex Lab A+B)
Array Tomography (Location: ATC microscope room 116)
Image Processing (Location: ATC Courtyard room)
ATUMtome (Location: ATC microscope room 116)
15:00-16:30 Workshops/Panel Discussion

Panel Discussion: Large Data Handling
16:30-16:45

Coffee Break

Session 2
The challenges of sample diversity
Chair: Chris Guerin, VIB Ghent, Belgium

16:45-17:30

What’s coming through the door now? Customizing sample preparation for volume EM in an imaging core facility
Chris Guerin, VIB Ghent, Belgium

17:30-18:00

The Acquisition of 3D datasets at ultrastructural level following light microscopy image acquisition : few examples
Christel Genoud, Friedrich Miescher Institute for Biomedical Research, Switzerland

18:00-18:30

3D-EM in multiuser environment: from cultured cells to plant roots and human carotid arteries
Eija Jokitalo, University of Helsinki, Finland

18:30-19:00

New Tools for Correlated Microscopies and Serial Blockface Scanning EM
Thomas Deerinck, National Center for Microscopy and Imaging Research (NCMIR), University of California, San Diego, United States of America

19:00-19:15 Correlated Serial-Section Light and Electron Microscopy of the cell-cell recognition process during zipping in Drosophila melanogaster
Mandy Börmel, University of Zurich, Switzerland
19:15-19:30 3D subcellular imaging of the Hepatitis A Virus (HAV)-induced replication factories
Inés Romero Brey, University of Heidelberg, Germany
19:45 Bus downtown
Day 3 - Tuesday 15 March 2016
TimeSpeaker
Session 3
From large volume imaging to data analysis and processing
Chair: Anna Kreshuk, IWR and Heidelberg Collaboratory for Image Processing (HCI), University of Heidelberg, Germany
09:00-09:45 EM image processing: ilastik news and algorithms with priors
Anna Kreshuk, IWR and Heidelberg Collaboratory for Image Processing (HCI), University of Heidelberg, Germany
09:45-10:00

3D Electron Microscopy of Biological Samples
Minh Huynh, Australian Centre for Microscopy & Microanalysis, The University of Sydney, Australia 

10:00-10:45

Coffee Break and Poster Session

10:45-11:15

Microscopy Image Browser: an open-source platform for segmentation and analysis of multidimensional datasets
Ilya Belevich, Institute of Biotechnology, University of Helsinki, Finland

11:15-11:45

Ec-CLEM: an open source plugin for CLEM image and volume registration
Perrine Paul-Gilloteaux, INRIA, Rennes, France

11:45-12:00

From a biological sample to high-quality digital image representation: avoiding and restoring image artifacts through proper acquisition and image restoration
Joris Roels, Ghent University - iMinds - VIB, Belgium 

12:00-13:00 Lunch and Poster Session
Even Numbers
13:00-14:30

Workshops

FIB-SEM (Location: EMCF room 309)
Sample Preparation (Location: EMCF room 305A)
3View (Location: ATC Flex Lab A+B)
Array Tomography (Location: ATC microscope room 116)
Image Processing (Location: ATC Courtyard room)
ATUMtome (Location: ATC microscope room 116)

14:30-16:00

Workshops/Panel Discussion

Panel Discussion: Shared experiences in CLEM

16:00-16:15

Coffee Break

Session 4
How 3D CLEM connects to other 3D Imaging Techniques
Chair: Peter O’Toole, University of York, United Kingdom

16:15-17:00

Making Light work of 3D Bioimaging
Peter O’Toole, University of York, United Kingdom

17:00-17:30

Correlative microscopy-spectroscopy studies of bone materials
Silke H. Christiansen, HZB, Germany

17:30-18:00

Structural analysis of multicellular organisms with cryo-FIB and cryo-electron tomography
Jan Harapin, University of Zurich, Switzerland

18:00-18:15

Shaping the Plant Endoplasmatic Reticulum
Maike Kittelmann, Oxford Brookes University, United Kingdom

18:15-18:30 X-Ray Microscopy in the life science
Rosy Manser, Carl Zeiss Microscopy GmbH, Germany
18:30-20:30 Conference Special Dinner
Bus to ISG and downtown
Day 4 - Wednesday 16 March 2016
TimeSpeaker
Session 5
Diversity of applications in biology
Chair: Richard Webb, University of Queensland, Australia
09:00-09:45

New Sample Processing for Serial Block-Face Scanning Electron Microscopy
Richard Webb, University of Queensland, Australia

09:45-10:00

Genetically encoded miniSOG probes for Drosophila cell labelling, neuronal volume imaging using Electon Microscopy and correlative X-ray microscopy
Julian Ng, University of Cambridge, United Kingdom

10:00-10:45

Coffee Break and Poster Session

10:45-11:15

3D CLEM: Past, Present and Future of Correlative Microscopy
Roger Wepf, ETH Zurich, Switzerland

11:15-11:45

Large-scale EM  (“nanotomy”) & correlative microscopy
Ben Giepmans, UMCG, The Netherlands

11:45-12:00

Myelination in the CNS
Mikael Simons, Max Planck Institute for Experimental Medicine, Germany

12:00-13:00 Lunch and Anonymous Feedback
13:00-14:30 Workshops

FIB-SEM (Location: EMCF room 309)
Sample Preparation (Location: EMCF room 305A)
3View (Location: ATC Flex Lab A+B)
Array Tomography (Location: ATC microscope room 116)
Image Processing (Location: ATC Courtyard room)
ATUMtome (Location: ATC microscope room 116)
14:30-16:00

Workshops/Panel Discussion

Panel Discussion Facility Implementation

16:00

Departure