The dynamic actin cytoskeleton and associated motor proteins of the myosin family generate forces to shape cellular membranes. The actin cytoskeleton thereby mediates all cell shape changes underlying embryogenesis, differentiation, cell motility, cell division as well as intracellular vesicle transport and pathogen motility. This course will introduce the participants to the most modern single-filament, single-molecule imaging techniques and demonstrate their utility in in vitro reconstituted assays as well as in live cells. We will also demonstrate how to use computational modelling to construct physical models of mechanisms of force generation.
Applicants do not need any prior knowledge of the techniques. The course is geared towards PhD students and early stage postdocs.
- live cell imaging (yeast and mammalian cells)
- single filament imaging in in vitro reconstituted systems (TIRF)
- advanced microscopy (FRET, super resolution imaging)
- computational modeling
Participants will use cutting-edge imaging technologies and quantitative analyses to gain mechanistic insights into mechanisms of actin-mediated force generation in vitro as well as in live cells.