Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify different from heterogeneous samples, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. Improvements in assay technology are now enabling scientist to detect mRNAs in cells using flow cytometry. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.
The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry is of advantage, but not a must.
• In-depth principles of Flow Cytometry
• Quality assessment and instrument validation
• Staining of tissues with cell surface antigens
• Staining cell cycle profiles in fixed and live cells
• Assessing proliferation and cell division
• Measuring RNA with FISH
• The beauty and burdens of fluorescence proteins in Flow Cytometry
• Analysing data from Flow Cytometers
Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.
This course will happen with the generous support from Becton Dickinson and ThermoFisher providing access to Flow Cytometers, reagents, and whole assay units. We are very grateful to Treestar giving us access to FlowJo for the duration of the course.