Location & dates EMBL Heidelberg, Germany 5 - 6 Apr 2017
Deadlines Application closed
organiser_eppendorf

Course Programme

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Day 1 - 05 April 2017
TimeSpeaker
09:00-09:15

Welcome remarks
Sandra Stobrawa - Eppendorf AG, Germany

09:15-09:45

Microinjection: Handling of the microinjection devices
Sandra Stobrawa - Eppendorf AG, Germany

09:45-10:30

Production of transgenic mice via DNA microinjection
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

10:30-10:45

Coffee break

10:45-11:15

Site-specific deletion / insertion / integration via microinjection into zygotes
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

11:15-12:00

Introduction into the usage of the microscope and correct contrast techniques
Werner Wittke - Leica Microsystems CMS GmbH, Germany

12:00-13:00 Lunch in the canteen
13:00-13:30

Demonstration
- Usage of the microscope and correct contrast techniques

13:30-17:00

Demonstration and hands-on
- Microinjection into pronuclei and cytoplasm of mouse zygotes

Day 2 - 06 April 2017
TimeSpeaker
09:00-09:30 Piezo-assisted micromanipulation 
Sandra Stobrawa - Eppendorf AG, Germany
09:30-10:30

Production of genetically modified mice via ES cell transfer into early embryos
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

10:30-10:45

Coffee break

10:45-12:15

Demonstration and Hands-on
- ES cell transfer into blastocysts

12:15-13:15

Lunch in the canteen

13:15-16:30

Hands-on
- ES cell transfer into blastocysts
- Piezo-assisted ES cell transfer into 8-cell embryo and blastocysts

16:30-17:00 Discussion and Feedback