Location & dates EMBL Heidelberg, Germany 27 - 31 Mar 2017
Deadlines Application closed

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

Got something to say? Share it with #EMBLEVs

Day 1 - Monday 27 March 2017
TimeSpeaker

EV isolation
Chairs:
An Hendrix
Jacqueline Dreyer-Lamm

09:00 - 10:00

Welcome and Introduction "Breaking the ice"
An Hendrix - Ghent University
Jacqueline Dreyer-Lamm - EMBL Heidelberg

10:00 - 10:45

Lecture:
Biogenesis of extracellular vesicles

Guillaume Van Niel - Institut Curie

10:45 – 11:00 Coffee break
11:00 – 11:45

Lecture:
Heterogeneity in EV isolation and characterization

An Hendrix - Ghent University

11:45 – 12:30

Lecture:
Impact of pre-analytical factors on EV isolation
Cecilia Lässer - University of Gothenburg

12:30 – 13:30

Lunch

13:30 – 14:00

Practical:
Preparation of the samples by differential ultracentrifugation (1h at 100,000g)
An Hendrix - Ghent University and Esther Nolte-'t-Hoen- University of Utrecht

14:00 - 15:00

Lecture:
EV isolation by differential ultracentrifugation and density gradient centrifugation

An Hendrix - Ghent University and Esther Nolte-'t-Hoen - University of Utrecht

15:00 – 16:30

Practical:
Fluorescent labeling of EVs
An Hendrix - Ghent University and Esther Nolte-'t-Hoen - University of Utrecht

16:30 – 17:30

Practical:
Isolation of EVs using Optiprep density gradient centrifugation
An Hendrix - Ghent University and Esther Nolte-'t-Hoen - University of Utrecht

17:30 – 18:00

Short presentations by participants

18:00 – 19:00 Dinner at EMBL
19:00 – 21:00

Poster Session (even numbers)

Day 2 - Tuesday 28 March 2017
TimeSpeaker

EV isolation and characterization
Chair:
Guillaume Van Niel

09:00 – 09:15

Outline of the day

09:15 – 09:30

Lecture:
Introduction to size exclusion chromatography
Julien Muzard - Izon Science

09:30 – 11:30

Practical:
Isolation of EVs using size exclusion chromatography
Jan Van Deun - Ghent University and Sten Libregts - University of Utrecht

Technical introduction
Particle Metrix: Sascha Raschke - Particle Metrix
Nanosight: Rolf Nitzsche - Malvern Instruments GmbH
qNano: Julien Muzard - Izon Science

11:30 – 12:00

Preparation of Group activity (on day 4)

12:00 – 13:00 Lunch break
13:00 – 13:30

Practicals: (one participant from each group)
Collect fractions from density gradients and centrifuge (1h at 100,000g)
An Hendrix and Jan Van Deun - Ghent University

13:30 – 14:00

Practicals: (one participant from each group)
Cell culture
Sten Libregts - University of Utrecht

13:00 – 17:30

Practicals:

NTA
An Hendrix - Ghent University and Rolf Nitzsche - Malvern Instruments GmbH

TRPS
Esther Nolte-'t-Hoen and Sten Libregts - University of Utrecht and Julien Muzard - Izon Science

NTA
Jan Van Deun - Ghent University and Sascha Raschke - Particle Metrix

17:30 – 18:00

Short presentations by participants

18:00 – 19:00

Dinner at EMBL

19:00 – 21:00

Poster session (odd numbers)

Day 3 - Wednesday 29 March  2017
TimeSpeaker
EV characterization and function
Chair:
An Hendrix
09:00 – 09:15

Outline of the day

09:15 – 10:00

Lecture:
Isolation of EVs from different biofluids
Olivier De Wever - Ghent University

10:00 – 10:15 Coffee break
10:15 – 11:00

Lecture:
EV characterization by transmission electron microscopy and western blot
Guillaume Van Niel - Institut Curie

11:00 – 12:00

Group Activity:
Tour of electron microscopy core facility with Q&A session
Jacqueline Dreyer and Yvonne Yeboah - EMBL Heidelberg

12:00 – 13:00

Lunch

13:00 – 19:00

Practicals:

Electron microscopy

Guillaume Van Niel and Roberta Palmulli - Institut Curie

EV characterization by flow cytometry and Treatment of cells with fluorescent labeled EVs
(analysis on day 4)
Esther Nolte-'t-Hoen and Sten Libreghts - University of Utrecht

Implementation of the EV-TRACK knowledgebase
An Hendrix and Jan Van Deun - Ghent University

19:00 - 20:00 Dinner at EMBL
Day 4 - Thursday 30 March 2017
TimeSpeaker

EV isolation, characterization and function
Chair:
Olivier De Wever

09:00 – 09:15 Outline of the day
09:15 – 10:00

Lecture:
Quantitative and qualitative EV analysis by flow cytometry
Marca Wauben - University of Utrecht

10:00 – 12:30

Pratical:
Quantification of fluorescent labeled EVs and analysis of EV transfer by flow cytometry

Sten Libreghts and Esther Nolte-'t-Hoen - University of Utrecht

10:00 – 12:30

Preparation of Group activity (on day 4)

12:30 – 13:30

Lunch

13:30 – 14:30

Group Activity/Presentation
Identification of benefits and limits of EV isolation and characterization methods

14:30 – 16:30

Group Activity:
Discussion of results
An Hendrix - Ghent University, Esther Nolte-'t-Hoen - University of Utrecht and Guillaume Van Niel - Institut Curie

16:30

Free Evening

17:30

Optional - Guided Tour of Heidelberg

Day 5 - Friday 31 March 2017
TimeSpeaker

EV composition: RNA, protein, lipid
Chairs:
An Hendrix, Esther Nolte-'t-Hoen, Guillaume Van Niel

09:15 – 10:00

Outline of the day

09:15 – 10:00

Lecture:
Validation of the EV transcriptome by RT-qPCR
Jo Vandesompele - Ghent University

10:00– 10:45

Lecture:
Transcriptomics
Pieter Mestdagh - Ghent University

11:15 – 11:30

Coffee break

11:30 – 12:15

Lecture:
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA
Esther Nolte-'t-Hoen - University of Utrecht

12:15– 13:15

Lunch break

13:15 – 14:15 Lecture:
Lipidomics
Johan Swinnen - KU Leuven
14:15 – 14:45 Lecture:
Proteomics
An Hendrix - Ghent University
14:45 - 15:15 Coffee break
15:15 – 16:00

Group Activity:
RNA, Protein and lipid
Questions and answers; Do's and Don't's

16:00 – 16:30

Wrap-up and conclusions

16:30 – 17:00

Poster awards and goodbye

17:00

Bus to ISG Hotel and dinner downtown

19:00

Course dinner downtown