Location & dates EMBL Heidelberg, Germany 17 - 22 Sep 2017
Deadlines Application closed
Organiser_ThermoFisher

Course Overview

This course will provide hands-on training in genome editing and cell engineering in mammalian cells using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts/knock-ins, and target validation using the most current technologies.

Audience

This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell lines using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.

Modules/Resources

During this course you will learn:

  • How to design target specific CRISPR/Cas9 tools
  • Generation of guide RNA (gRNA)
  • Transfection optimization for efficient gene editing
  • Generation of knock-out and knock-in cell lines
  • Validation of genome-edited cell lines

Learning Outcomes

After this course you should be able to:

  • Design CRISPR based editing tools for your target gene of interest
  • Choose the right format of gene editing tool and delivery reagent for your cell type of interest
  • Edit, screen and validate the engineered cell lines

This course is co-organised by Thermo Fisher.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • Becton Dickinson logo
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Illumina logo
  • Sartorius
  • ThermoFisher logo

Associate partners

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  • Nikon logo
  • Sanofi logo