EMBL Courses and Conferences during the Coronavirus pandemic
With the onsite programme paused, many of our events are now being offered in virtual formats.
Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.
More information for participants of events at EMBL Heidelberg can be found here.
Course Overview
The goal of this course is to teach the fundamentals of NGS library preparation and validation using the Illumina TruSeq stranded total RNA sample preparation kit. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the preparation of libraries from total RNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for total RNA sequencing data analysis.
Audience
This course is directed towards biologists, scientists and technicians who want to apply RNA sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.
Modules/Resources
During this course we will focus on the following aspects:
- Best practices and tips and tricks for TruSeq stranded total RNA library preparation
- Best practices of sequencing library validation
- Introduction to RNA sequencing data analysis
Learning Outcomes
After this course you should be able to:
- Prepare libraries from total RNA using the Illumina TruSeq stranded total RNA sample preparation kit
- Quality control and quantify the libraries
- Understand the basics of RNA sequencing data analysis
This course is co-organised with illumina.
