Location & dates EMBL Heidelberg, Germany 2 - 5 May 2017
Deadlines Application closed
organiser_illumina

Course Overview

The goal of this course is to teach the fundamentals of NGS library preparation and validation using the Illumina TruSeq stranded total RNA sample preparation kit. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the preparation of libraries from total RNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for total RNA sequencing data analysis. 

Audience

This course is directed towards biologists, scientists and technicians who want to apply RNA sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

During this course we will focus on the following aspects:

  • Best practices and tips and tricks for TruSeq stranded total RNA library preparation
  • Best practices of sequencing library validation
  • Introduction to RNA sequencing data analysis

Learning Outcomes

After this course you should be able to:

  • Prepare libraries from total RNA using the Illumina TruSeq stranded total RNA sample preparation kit
  • Quality control and quantify the libraries
  • Understand the basics of RNA sequencing data analysis

This course is co-organised with illumina.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • Becton Dickinson logo
  • Boehringer Ingelheim logo
  • GSK logo
  • Illumina logo
  • Sartorius
  • ThermoFisher logo

Associate partners

  • Eppendorf logo
  • Merck logo
  • Nikon logo
  • Sanofi logo