Location & dates EMBL Heidelberg, Germany 15 - 19 May 2017
Deadlines Application closed

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.

Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.

More information for participants of events at EMBL Heidelberg can be found here.

Course Overview

The goal of this course is to teach the fundamentals of NGS library preparation and validation using enrichment based tests like the Nextera / TruSight Rapid Capture assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and quantify the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of Enrichment based sequencing results using standard Illumina software tools like MiSeq Reporter and VariantStudio.


This course is directed towards molecular biologists, scientists and technicians who want to apply Enrichment based targeted sequencing in their lab. Knowledge of next generation sequencing technology is desirable but not essential.


During this course we will focus on the following aspects:

  • Wet lab training and best practices for Nextera / TruSight Rapid Capture library preparation
  • Best practices of sequencing library validation and quantification
  • Custom assay design using DesignStudio
  • Introduction to Enrichment based sequencing data analysis and interpretation using Illumina MiSeq Reporter and VariantStudio software

Learning Outcomes

After this course you should be able to:

  • Prepare libraries from genomic DNA using Nextera / TruSight Rapid Capture approaches
  • Quality control and quantify libraries
  • Understand the basics of assay design using DesignStudio
  • Analyse and interpret data using Illumina MiSeq Reporter and VariantStudio software

This course is co-organised with illumina.

Additional course dates


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • 10x Genomics_120x82
  • Becton Dickinson logo
  • Bio-Rad logo
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Milteniy Biotec logo
  • NetApp logo
  • Stilla logo
  • ThermoFisher logo

Associate partners

  • Merck logo
  • New England Biolabs logo
  • Nikon logo
  • Promega logo
  • Roche logo
  • Sanofi logo
  • Sartorius logo