Location & dates EMBL Heidelberg, Germany 15 - 19 May 2017
Deadlines Application closed
organiser_illumina

Final Programme

Day 1 - Monday 15 May 2017
TimeTopic
10.30 - 11.00 Registration & Hand in DNA Samples (Optional)
11.00 - 11.15 Official Course Start - Welcome
11.15 - 12.00 Introduction
12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 14.15 DNA quantification
14.15 - 15.00 Tagmentation
15.00 - 15.30 Tegment Clean Up
15.30 - 15.45 Coffee break  /  Tegment Clean Up
15.45 - 16.15 Tegment Clean Up
16.15 - 17.15 Set up PCR
17.15 - 18.00 Start Fragment Analyzer
18.00 - 19.00 Dinner at the EMBL Canteen
Day 2 - Tuesday 16 May 2017
TimeTopic
8.15 - 9.30 Clean up PCR
9.30 - 10.00 Start Fragment Analyzer
10.00 - 10.30 Coffee break
10.30 - 11.00 Quantify libraries
11.00 - 11.30 Pool samples and concentrate
11.30 - 12.00 Set up 1st Hybridization
12.00 - 13.00 Lunch at the EMBL Canteen  /  Hybridization
13.00 - 14.00 Troubleshooting & Best Practices  /  Hybridization
14.00 - 14.30 Add Beads
14.30 - 15.00 Set up 1st Wash
15.00 - 15.30 Coffee Break /  Wash
15.30 - 16.00 Set up 2nd Wash
16.00 - 16.30 Illumina Tools  /  Wash
16.30 - 17.00 Elution
17.00 - 18.00 Set up Hybridization
18.00 - 19.00 Dinner at the EMBL Canteen
Day 3 - Wednesday 17 May 2017
TimeTopic
8.15 - 9.00 Gene Core Lab Tour
9.00 - 9.30 Add Beads
9.30 - 10.00 Set up 1st Wash
10.00 - 10.30 Library Validation  /  Wash
10.30 - 11.00 Set up 2nd Wash
11.00 - 11.30 Coffee Break /  Wash
11.30 - 12.00 Elution
12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 14.00 Clean Up
14.00 - 14.30 Set Up PCR
14.30 - 15.00 Design Studio  /  PCR
15.00 - 15.30 Coffee Break /  PCR
15.30 - 16.30 Clean up PCR
16.30 - 17.45 Qbit Measurement & Start Fragment Analyzer
from 17.45 Free Evening
Day 4 - Thursday 18 May 2017
TimeTopic
9.00 - 10.30 Analysis of Library Validation
10.30 - 11.00 Coffee break
11.00 - 12.00 Introduction to the MiSeq and NextSeq
12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 15.15 Set up a Sequencing Run
15.15 - 15.45 Coffee break 
15.45 - 16.45 MiSeq/NextSeq Run Monitoring
16.45 - 17.00 Coffee break
17.00 - 18.00 TruSight / NRC Data Output Files
18.00 - 19.00 Dinner at the EMBL Cafeteria
Day 5 - Friday 19 May 2017
TimeTopic
9.00 - 9.30 Quiz 
9.30 - 10.30 MiSeq Reporter / Basespace
10.30 - 11.00 Coffee break
11.00 - 12.00 Variant Studio
12.00 - 12.30 Feedback & Wrapup
12.30 - 13.30 Lunch at the EMBL Canteen