Location & dates EMBL Heidelberg, Germany 10 - 15 Dec 2017
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Programme

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Day 1 - Sunday 10 December 2017
TimeSpeaker
14.00 - 14.30 Registration & Welcome
14.30 - 15.00

Welcome address, introduction, overview of the course

15.00 - 16.00

CLEM coming of age: an introduction and some history
Paul Verkade, University of Bristol, UK

16.00 - 16.20 Coffee break
16.20 - 18.45

Flash Talks, Meet the Participants

19.00 Pizza dinner and drinks in the ATC Rooftop Lounge
Day 2 - Monday 11 December 2017
TimeSpeaker
09.00 - 09:15

Safety briefing

09.15 - 10.15

Lecture: Endocytosis as a paradigm for understanding membrane remodeling at the cell surface using CLEM
Ori Avinoam, Weizmann Institute of Science, Israel

10.15 - 10.30

Coffee Break

10.30 - 11.15

Lecture: Technical aspects of the method for plastic sections
Paolo Ronchi, EMBL Heidelberg, Germany

11.15 - 12.00 Lecture: Introduction to Cryo CLEM
Martin Schorb, EMBL Heidelberg, Germany
12.00 - 13.00 Lunch
13.00 - 18.00

Practicals

18.00 - 18.45

Tips and tricks to adapt on-section CLEM to diverse biological questions
Paolo Ronchi, EMBL Heidelberg, Germany

19.00 Dinner
Day 3 - Tuesday 12 December 2017
TimeSpeaker
09.00 - 10.00 Lecture: History of Cryo-CLEM
Anna Sartori-Rupp, Institut Pasteur, France
10.00 - 10.30 Solutions for CLEM and recent advances in Cryo EM
Manuel Reinhard, JEOL, Germany
10.30 - 11.00

Coffee Break

11.00 - 11.45

Lecture: Tricks and pitfalls in ultramicrotomy
Helmut Gnaegi, Diatome, Switzerland

11.45 - 12.15 EM sample preparation solutions
Thorben Kurz, Leica Microsystems, Germany
12.15 - 13.15

Lunch

13.15 - 19.00 Practicals
19.00 Dinner
Day 4 - Wednesday 13 December 2017
TimeSpeaker
09.00 - 10.00

Lecture: Introduction to transformations and registrations
Martin Schorb, EMBL Heidelberg, Germany and Perrine Paul-Gilloteaux, University of Nantes, France

10.00 - 10.30 Coffee Break/Discussion
10.30 - 12.00

Remote demo session on Serial EM
Martin Schorb, EMBL Heidelberg, Germany

12.00 - 13.00 Lunch
13.00 - 16.00

Icy Software demo/practical
Perrine Paul-Gilloteaux, University of Nantes, France and Martin Schorb, EMBL Heidelberg, Germany

16.00 - 17.00

Application talk: The development of high-throughput fluorescent barcoding CLEM protocol for budding yeast
Yury Bykov, Medical Research Council, UK

17:30 Departure for Christmas markets and dinner downtown
Day 5 - Thursday 14 December 2017
TimeSpeaker
09.00 - 10.00 Applying CLEM to study pathogens
Petr Chlanda, University of Heidelberg, Germany
10.00 - 10.45

Volume CLEM - perfect timing at the perfect place
Pedro Machado, EMBL Heidelberg, Germany

10.45 - 11.00 Coffee Break
11.00 - 12.00 Site Specific Cryo-FIB Preparation Aimed at in situ Cryo-Electron Tomography
Julia Mahamid, EMBL Heidelberg, Germany
12.00 - 12.30 Towards a workflow for cryoTEM preparation in structural biology
Alexander Rigort, FEI, Germany
12.30 - 13.30 Lunch
13:30 - 19.00 Open lab
19:00 Dinner
Day 6 - Friday 15 December 2017
TimeSpeaker
09.00 - 10.00

Cell Biology meets Synthetic Biology
Paul Verkade, University of Bristol, UK

10.00 - 12.30

Open lab

12.30 - 13.00

Wrap up/feedback

13.30 Packed lunch and farewell