Location & dates EMBL Heidelberg, Germany 9 - 13 Oct 2017
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Programme

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Day 1 - Monday 9 October 2017
TimeSpeaker
09.00 - 09.30

Welcome & Introductions
Jacqueline Dreyer-Lamm - EMBL Heidelberg, Germany

09.30 - 10.45

Lecture: Flow Cytometry
Malte Paulsen - EMBL Heidelberg, Germany

10.45 - 11.00 Coffee break
11.00 - 12.00

Practical: Single Cell Sorting
Malte Paulsen - EMBL Heidelberg, Germany

12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 13.30

Lecture: SiMSen-Seq workflow
Anders Stahlberg - Sahlgrenska Academy at University of Gothenburg, Sweden
Tony Godfrey - Boston University School of Medicine, USA

13.30 - 15.00 Practical: DNA extraction from Plasma
15.00 - 15.15 Coffee break
15.15 - 18.15 Practical: Library Preparation
18.15 - 19.00

Dinner at EMBL

19.00 - 21.00 Poster Session I
Day 2 - Tuesday 10 October 2017
TimeSpeaker
09.00 - 09.15 Outline
09.15 - 10.30

Lecture:  Liquid Biopsies in the clinical context High-fidelity sequencing
Tony Godfrey - Boston University School of Medicine, USA

10.30 - 10.45

Coffee break

10.45 - 12.00

Practical: Clean-Up and quality control

12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 13.30

Practical: Single-cell RT

13.30 - 14.30

Lecture:  Introduction to ddPCR
Francisco Bizouarn - BioRad, Spain

14.30 - 15.00 Practical: Single-cell qPCR
15.00 - 15.15

Coffee break

15.15 - 17.00

Practical: ddPCR
Francisco Bizouarn - BioRad, Spain

17.00 - 18.00

Lecture:  Introduction into QuantaSoft, Start reading the experiment
Francisco Bizouarn - BioRad, Spain

18.00 - 19.00

Dinner at EMBL

19.00 - 21.00

Poster Session II

Day 3 - Wednesday 11 October 2017
TimeSpeaker
09.00 - 09.15 Outline
09.15 - 10.15

Practical: Data analysis with QuantaSoft
Francisco Bizouarn - BioRad, Spain

10.15 - 10.30 Coffee break
10.30 - 11.30 Practical: Data analysis with QuantaSoft
Francisco Bizouarn - BioRad, Spain
11.30 - 12.30

Lecture:  Effect of pre-analytical factors on the measurement of Evs in Liquid Biopsies
An Hendrix - Ghent University, Belgium

12.30 - 13.30 Lunch at the EMBL Canteen
13.30 - 14.30

Lecture: Clinical validation of droplet-based procedures in the context of liquid biopsies
Valérie Taly - Paris Descartes University, France

14.30 - 15.30

Lecture: Molecular Analyzes of CTCs: challenges and opportunities of single cell-based approaches
Rui Neves - Hospital Centre of the Heinrich-Heine University Düsseldorf, Germany

15.30 - 16.00

Coffee break

16.00 - 17.00

Lecture: Single-Cell Analysis Technologies and Applications
Anders Stahlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

from 17.00

Free evening

Day 4 - Thursday 12 October 2017
TimeSpeaker
09.00 - 09.15

Outline

09.15 - 10.30

Lecture: Comparison of exosome extraction kits Exosome content miRNA, isomiRs Biomarker for sepsis
Michael Pfaffl - Technical University of Munich, Germany

10.30 - 10.45

Coffee break
10.45 - 12.00 Lecture: Protein detection in Liquid Biopsies
Ulf Landegren - Uppsala University, Sweden
12.00 - 13.00 Lunch at the EMBL Canteen
13.00 - 14.00

Lecture: Workflow of PLA etc. in Liq. Biopsies
Ulf Landegren - Uppsala University, Sweden

14.00 - 15.00

Lecture: Potential clinical utility of CTC and ctDNA analysis
Ged Brady - The University of Manchester, United Kingdom

15.00 - 16.00 Lecture: CTC, Single-Cell
Mikael Kubista - Laboratory of Gene Expression, Czech Republic
16.00 - 17.15

Lecture: Single-cell analysis
Data analysis

17.15 - 18.30

Q&A Session / Ask the expert

19.00

Course Dinner downtown

Day 5 - Friday 13 October 2015
TimeSpeaker
09.00 - 09.15 Outline
09.15 - 10.15

Lecture: Single-cell analysis / Data analysis
Mikael Kubista - Laboratory of Gene Expression, Czech Republic

10.15 - 10.45

Coffee break

10.45 - 12.15

Practical: SiMSen-Seq / Data analysis
Anders Stahlberg - Sahlgrenska Academy at University of Gothenburg, Sweden
Stefan Filges - Sahlgrenska Academy at University of Gothenburg, Sweden

12.15 - 13.15 Lunch at the EMBL Canteen
13.15 - 15.30

Practical: SiMSen-Seq / Data analysis
Anders Stahlberg - Sahlgrenska Academy at University of Gothenburg, Sweden
Stefan Filges - Sahlgrenska Academy at University of Gothenburg, Sweden

15.30 - 16.00 End of Course