Non-coding RNAs (ncRNAs) continue to represent a significant, ubiquitous and ever-growing class of regulatory RNAs that are principally involved in control of many cellular processes of most organisms, regardless if bacterial or eukaryotic. Mechanisms of their function are not yet comprehensively understood. However, it is now clear that they also play considerable roles in pathology of organisms, which makes them clinically relevant too.
The course will focus on methods enabling characterization of cellular content and localization of ncRNAs as well as providing insights into their function. In particular, we will teach methods allowing investigation of these molecules in all organisms, not only in human or mouse. The practical part will include detection and quantification of ncRNAs, data analysis and integration as well as correlation of data obtained by various methods.
The course is open to students at an advanced stage of their post-graduate work (Ph.D training) and post-doctoral fellows.
During the course we will pay particular attention to following aspects:
a) explanation and practicing of different approaches used for ncRNA detection & characterization of their abundance;
b) detailed troubleshooting for all steps required to characterize ncRNA content and profiles;
c) comparison of analyses' strategies and evaluation of obtained data;
d) experimental approaches for the identification of ncRNA 'targets' and for the functional validation of their activity.
This course is kindly supported by the Gemomics Core Facility.