Location & dates EMBL Heidelberg, Germany 4 - 8 Jun 2018
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Programme

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Day 1 - Monday 4 June 2018
TimeSpeakerLocation
08:00 - 08:30

Arrival

Flex Lab A & B

08:30 - 10:00

Welcome & Introductions

Flex Lab A & B
10:00 - 12:00

Lecture: Fluidics and Optics
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A & B
12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Lecture: Fluorescence & PMTs/Detectors
Steffen Schmitt - German Cancer Research Center, Germany
Lecture: Data generation, Data display
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A & B

15:00 - 19:00

Practical Session:
Assessing Instrument performance knoweldge interlude:
What is sensitivity How to evaluate performance easily every day    
Practical Session:
Absolute Counting and Proliferation

Flex Lab A & B

19:00 - 20:00

Dinner

EMBL Canteen

Day 2 - Tuesday 5 June 2018
TimeSpeakerLocation
09:00 - 12:00

Lecture: Fluorescence Spillover and compensation
Cora Chadick - EMBL Rome, Italy
Lecture: Experimental design for multi-colour Flow
Andrea Engel - BD, Germany

Flex Lab A & B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Practical Session:
Group 1: Measuring Multi-colour surface staining 
Group 2: Data analysis with FlowJo Session 1

Flex Lab A & B

15:00 - 15:30

Break

Flex Lab A & B

15:00 - 17:30

Practical Session:
Group 2: Measuring Multi-colour surface staining
Group 1: Data analysis with FlowJo Session 1

Flex Lab A & B

17:30 - 18:30

Lecture: Tips and tricks for intracellular stainings and fixation
Diana Ordonez - EMBL Heidelberg, Germany

Flex Lab A & B

18:30 - 19:30

Dinner

EMBL Canteen

Day 3 - Wednesday 6 June 2018
TimeSpeakerLocation
09:00 - 10:00

Lecture: The essentials of gating, FMO and proper internal gating controls
Steffen Schmitt - German Cancer Research Center, Germany

Flex Lab A & B

10:00 - 10:30

Break

Flex Lab A & B

10:30 - 12:00

Lecture: Cell Cycle Analysis
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A & B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Practical Session:
Group 1: Measuring univariate and bivariate cell cycle samples
Group 2: Data analysis with FlowJo Session 2

Flex Lab A & B

15:00 - 15:30

Break

15:30 - 17:30

Practical Session:
Group 2: Measuring univariate and bivariate cell cycle samples
Group 1: Data analysis with FlowJo Session 1

Flex Lab A & B

17:30 - 18:00

Q&A Session

Flex Lab A & B
18:00 - 19:00

Dinner

EMBL Canteen
Day 4 - Thursday 7 June 2018
TimeSpeakerLocation
09:00 - 10:00

Practical Session: Analysis cell cycle- FlowJo

Flex Lab A & B

10:00 - 10:30

Break

Flex Lab A & B

10:30 - 12:00

Practical Session:
Data analysis cell cycle + Users’ data-FlowJo

Flex Lab A & B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Lecture: Assessing Cell Death
Steffen Schmitt - German Cancer Research Center, Germany

Flex Lab A & B

14:00 - 15:30

Practical Session: Measuring apoptosis

Flex Lab A & B

15:30 - 17:00

Practical Session: Data analysis-FlowJo

Flex Lab A & B

17:00 - 21:00

Social activity & Downtown Dinner

Restaurant

Day 5 - Friday 8 June 2018
TimeSpeakerLocation
09:00 - 10:00

Lecture: The basics of cell sorting
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A & B

10:00 - 12:00

Practical Session: Cell sorting and reanalysis of sorted cells

Flow cytometry core facility

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Lecture: Workshop revisiting the basics of the last 3.5 days

Flex Lab A & B

14:00 - 14:30

Feedback and Good-bye

Flex Lab A & B

14:40

Bus to Train Station & Crown Plaza Hotel