Location & dates EMBL Heidelberg, Germany 10 - 11 Apr 2018
Deadlines Application closed
organiser_eppendorf

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Tuesday, 10 April 2018
TimeSpeakerLocation
09:00-09:15

Welcome remarks
Sandra Stobrawa - Eppendorf AG, Germany

Courtyard Room A+B

09:15-09:45

Microinjection: Handling of the microinjection devices
Sandra Stobrawa - Eppendorf AG, Germany

Courtyard Room A+B

09:45-10:30

Production of transgenic mice via DNA microinjection
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

Courtyard Room A+B

10:30-10:45

Coffee break

outside Courtyard Room A+B

10:45-11:15

Site-specific deletion / insertion / integration via microinjection into zygotes
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

Courtyard Room A+B

11:15-12:00

Introduction into the usage of the microscope and correct contrast techniques
Werner Wittke - Leica Microsystems CMS GmbH, Germany

Courtyard Room A+B

12:00-13:00

Lunch in the canteen

EMBL Canteen

13:00-13:30

Demonstration
- Usage of the microscope and correct contrast techniques

Training Lab A
13:30-17:00

Demonstration and hands-on
- Microinjection into pronuclei and cytoplasm of mouse zygotes

Training Lab A

18:30

Dinner downtown

Restaurant Hotel zum Ritter, Heidelberg Old Town

Day 2 - Wednesday, 11 April 2018
TimeSpeakerLocation
09:00-09:30

Piezo-assisted micromanipulation 
Sandra Stobrawa - Eppendorf AG, Germany

Courtyard Room A+B

09:30-10:30

Production of genetically modified mice via ES cell transfer into early embryos
Frank van der Hoeven - Transgenic Service, German Cancer Research Center, Germany

Courtyard Room A+B

10:30-10:45

Coffee break

outside Courtyard Room A+B

10:45-12:15

Demonstration and Hands-on
- ES cell transfer into blastocysts

Training Lab A

12:15-13:15

Lunch in the canteen

EMBL Canteen

13:15-16:30

Hands-on
- ES cell transfer into blastocysts
- Piezo-assisted ES cell transfer into 8-cell embryo and blastocysts

Training Lab A
16:30-17:00

Discussion and Feedback

Courtyard Room A+B