Location & dates EMBL Heidelberg, Germany 8 - 14 Apr 2018
Deadlines Application closed
organiser_EMBO_Course_2017

Programme

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Day 1 - Sunday, 8 April 2018
TimeSpeakerLocation
18:00 - 19:00 Welcome and introductions

ISG Hotel

19:00 - 20:00 Flash talks by participants

ISG Hotel

20:00

Dinner at ISG Hotel

ISG Hotel

Day 2 - Monday, 9 April 2018
TimeSpeakerLocation
09:00 - 10:00

Group activity/ Outline of the day
An Hendrix, Ghent University, Belgium

Flex Lab A & B

10:00 - 10:45

Lecture:
Heterogeneity in EV isolation and characterization
An Hendrix, Ghent University, Belgium

Flex Lab A & B

10:45 - 11:00

Coffee break

Flex Lab A & B

11:00 - 11:45

Lecture:
Extracellular vesicles diversity
Edit Buzas, Semmelweis University, Hungary

Flex Lab A & B

11:45 - 12:45

Lunch break

EMBL Canteen

12:45 - 13:00

Lecture:
Introduction to practicals

An Hendrix, Ghent University, Belgium

Flex Lab A & B

13:00 - 14:00

Practical:
EV Isolation/ Preparation of the samples by differential ultracentrifugation
An Hendrix, Ghent University, Belgium

Training Lab B

14:00 - 14:45

Lecture:
EV Isolation/ Fluorescent labeling of EVs
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Flex Lab A & B
14:45 - 15:00

Coffee break

Flex Lab A & B
15:00 - 15:30

Lecture:
Biogenesis of extracellular vesicles
Guillaume Van Niel, Institut Curie, France

Flex Lab A & B
15:30 - 18:00

Practical:
EV Isolation/ Fluorescent labeling and Isolation of EVs using Optiprep density gradient centrifugation
An Hendrix, Ghent University, Belgium
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Training Lab B
18:00 - 19:00

Dinner

EMBL Canteen

19:00 - 20:30

Poster session I

Top of Helix A

Day 3 - Tuesday, 10 April 2018
TimeSpeakerLocation
09:00 - 09:15

Outline of the day
Guillaume Van Niel, Institut Curie, France

Flex Lab A & B

09:15 - 10:15

Practical:
EV Isolation and Characterization/Collect fractions from density gradients and centrifuge
An Hendrix, Ghent University, Belgium
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Training Lab B

10:15 - 11:15

Practical:
EV Isolation and Characterization/Isolation of EVs using size exclusion chromatography
An Hendrix, Ghent University, Belgium
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Training Lab B

11:15 - 11:30

Coffee break

Flex Lab A & B

11:30 - 12:30

Lecture:
Impact of pre-analytical factors on EV isolation
Cecilia Lässer, Gothenburg University, Sweden

Flex Lab A & B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 17:00

Practical:
EV Isolation and Characterization/ NTA & TRPS & particle metrix
An Hendrix, Ghent University, Belgium
Esther Nolte-’t Hoen, Utrecht University, The Netherlands
Jan Van Deun, Ghent University, Belgium
Sten Libregts, Utrecht University, The Netherlands

Training Lab B

17:00 - 18:00

Group activity:
Data analysis

Training Lab B
18:00 - 19:00

Dinner

EMBL Canteen
19:00 - 20:30 Poster Session II

Top of Helix A

Day 4 - Wednesday, 11 April 2018
TimeSpeakerLocation
09:00 - 09:15

Outline of the day
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Flex Lab A & B

09:15 - 10:00

Lecture:
EV isolation, characterization and function/ Quantitative and qualitative EV analysis by flow cytometry
Marca Wauben, University of Utrecht, The Netherlands

Flex Lab A & B

10:00 - 10:15

Coffee break

Flex Lab A & B

10:15 - 11:00

Lecture:
EV characterization and function/EV characterization by transmission electron microscopy and western blot analysis
Guillaume Van Niel, Institut Curie, France

Flex Lab A & B

11:00 - 12:00

Group activity:
EV characterization and function/ Tour of electron microscopy core facility with Q&A session

Flex Lab A & B
Electron Microscopy Core Facility
12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 19:00

Practical:
EV characterization and function/ Electron Microscopy
Guillaume Van Niel, Institut Curie, France
Roberta Palmulli, Institut Curie, France

Training Lab B

13:00 - 19:00

Practical:
EV characterization and function/ Treatment of cells with fluorescent labeled Evs
Esther Nolte-’t Hoen, Utrecht University, The Netherlands
Sten Libregts, Utrecht University, The Netherlands

Training Lab B

13:00 - 19:00

Practical:
EV characterization and function/ Implementation of EV-TRAQ
An Hendrix, Ghent University, Belgium
Jan Van Deun, Ghent University, Belgium

Training Lab B

19:00 - 20:00

Dinner

EMBL Canteen

Day 5 - Thursday, 12 April 2018
TimeSpeakerLocation
09:00 - 09:15 Outline of the day
Esther Nolte-’t Hoen, Utrecht University, The Netherlands

Flex Lab A & B

09:15 - 10:00 Lecture:
EV characterization and function/ Isolation of EVs from different biofluids
Olivier de Wever, Ghent University, Belgium

Flex Lab A & B

10:00 - 12:30

Practical:
Quantification of fluorescent labeled EVs and analysis of EV transfer by flow cytometry
Sten Libregts, Utrecht University, The Netherlands

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 15:15 Group activity:
Data analysis

Flex Lab A & B

15:15 - 17:00

Group activity:
Discussion of results

Flex Lab A & B
17:00

Free evening

Day 6 - Friday, 13 April 2018
TimeSpeakerLocation
09:00 - 09:15 Outline of the day
An Hendrix, Ghent University, Belgium

Flex Lab A & B

09:15 - 10:15

Lecture:
Validation of the EV transcriptome by RT-qPCR
Jo Vandesompele, Ghent University, Belgium

Flex Lab A & B

10:15 - 11:00

Lecture:
EV composition: RNA, protein, lipid/ Transcriptomics
Pieter Mestdagh, Ghent University, Belgium

Flex Lab A & B

11:15 - 11:30

Coffee break

Flex Lab A & B

11:30 - 12:30

Lecture:
EV composition: RNA, protein, lipid/ Lipidomics
Johan Swinnen, KU Leuven, Belgium

Flex Lab A & B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Lecture:
Isolation of extracellular vesicles: separation from lipoprotein particles and protein aggregates
Willem Stoorvogel, University of Utrecht, The Netherlands

Flex Lab A & B

14:30 - 15:30

Lecture:
Proteomics: exosomes, secretome and system biology
Suresh Mathivanan, La Trobe University, Australia

Flex Lab A & B

15:30 - 15:45

Coffee break

Flex Lab A & B

15:45 - 16:45 Practical:
Bioinformatic analysis of EV protein, RNA, lipid content
Suresh Mathivanan, La Trobe University, Australia

Training Lab B

16:45 - 18:00

Group activity:
Protein and lipid - Identification of Do's and Don't's

Flex Lab A & B

18:45

Course dinner downtown

Downtown restaurant

Day 7 - Saturday, 14 April 2018
TimeSpeakerLocation
09:00 - 09:15

Outline of the day
Guillaume Van Niel, Institut Curie, France

Flex Lab A & B

09:15 - 10:15

Lecture:
EVs nomenclature, subtypes, functions and biomedical applications
Identification of EV function by in vivo imaging
Michiel Pegtel, VU University Medical Center, The Netherlands

Flex Lab A & B

10:15 - 11:15

Lecture:
EVs nomenclature, subtypes, functions and biomedical applications
The therapeutic use of Evs
Bernd Giebel, Uniklinik Essen, Germany

Flex Lab A & B

11:15 - 11:30

Coffee break

Flex Lab A & B

11:30 - 12:00

Wrap-up and Conclusions & Poster awards

Flex Lab A & B

12:00

Packed Lunch and Departure

Flex Lab A & B