Location & dates EMBL Heidelberg, Germany 16 - 21 Sep 2018
Deadlines Application closed
Organiser_ThermoFisher

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Sunday 16 September 2018
TimeSpeaker
from 17.00 Arrival & Welcome at the ISG Hotel

Welcoming round of organizers, trainers, speakers at the ISG Hotel

Ice-breaker (Flash talks)

18:30 - 19:15

Introduction into CRISPR/Cas9
Sibylle Chantal Vonesch - EMBL Heidelberg, Germany

19:30

Dinner - ISG Hotel

Monday 17 September 2018
TimeSpeaker
9.00 - 9.30

Arrival & Welcome
Yvonne Yeboah, Elisabeth Zielonka - EMBL Heidelberg, Germany

9.30 - 10.15 Purification of Cas9 protein
Kim Remans - EMBL Heidelberg, Germany
10.15 - 10.45 Coffee Break
10.45 - 11.45

gRNA/Donor design (Intro to practical)
Thermo Fischer Scientific

11.45 - 12.30 Practical 1-1
Guide RNA Synthesis – PCR for IVT templates
12.30 - 13.30 Lunch in the EMBL Canteen
13.30 - 14.30

CRISPR issues and applications
Dana Carroll - The University of Utah, United States of America

14.30 - 15.00

Coffee break

15.00 - 16.30

Practical 1-2
PCR gel analysis and set up IVT

16.30 - 18.00

Practicals 2 & 3 in groups
Transfection of 293FT cells with gRNA & Cas9mRNA lipofection
Electroporation of 293FT with gRNA & Cas9protein
Guide RNA design task

18.00 - 19.00

Dinner in the EMBL Canteen

19.00 - 20.30

Practicals 2 & 3 in groups continues
Transfection of 293FT cells with gRNA & Cas9mRNA lipofection
Electroporation of 293FT with gRNA & Cas9protein
Guide RNA design task

Tuesday 18 September 2018
TimeSpeaker
9.00 - 9.45

Genome and epigenome editing using CRISPR-Cas system
Claudio Mussolino - Medical Center -University of Freiburg, Germany

9.45 - 10.00

Coffee break

10.00 - 11.00 Generation and validation of endogenously tagged cell lines
Birgit Koch - Max Planck Institute for Medical Research, Germany
11.00 - 12.30 Practicals in groups
a) Introduction to FACS by Malte Paulsen
    FACS sorting of knock in cells – single cell sort
b) Individual project discussion
12.30 - 13.30 Lunch in the EMBL Canteen
13.30 - 15.00 Practicals in groups continues
a) Introduction to FACS by Malte Paulsen
    FACS sorting of knock in cells – single cell sort
b) Individual project discussion
15.00 - 15.15 Coffee break
15.15 - 17.45

Practicals 1-3 & 4 in groups continues
IVT cleanup, quantitation and analysis of gRNA
Transfection of 293FT-eBFP cells using CRISPR/Cas9 for SNP (RNPs)

17.45 - 18.30

Dinner in the EMBL Canteen

18.30 - 20.30

Practicals 1-3 & 4 in groups
IVT cleanup, quantitation and analysis of gRNA
Transfection of 293FT-eBFP cells using CRISPR/Cas9 for SNP (RNPs)

Wednesday 19 September 2018
TimeSpeaker
09.00 - 09.30

CRISPR & HiBiT Protein Tag –A perfect team to investigate endogenous protein biology
Erik Bonke - Promega, Germany

09.30 - 10.00 High Efficiency Gene Editing using Programmable EnGen Ribonucleoprotein Complexes
Karl von Laer - New England Biolabs, Germany
10.00 - 10.15

Coffee break

10.15 - 12.00

Practical 5-1 & 5-2
GCD Cell Harvest and lysis, set up GCD PCR

12.00 - 13.00

Lunch in the EMBL Canteen

13.00 - 13.45 Mammalian cell engineering: The genome editing toolbox and considerations for use
Jon Chesnut - Thermo Fisher Scientific, USA
13.45 - 14.15 Practical 5-3
CR analysis, set up GCD re-annealing reaction
14.15 - 16.15 Practicals 5-4 & 5-5 in groups
Set up digestion for GCD, gel purification & sample submission for sanger sequencing
Gel analysis of GCD
16.15 - 16.30 Coffee break
16.30 - 18.30

TIDE - rapid, powerful and easy analysis of CRISPR experiments
Victor Dillard - Desktop Genetics, UK

18.30 - 19.30 Dinner in the EMBL Canteen
Thursday 20 September 2017
TimeSpeaker
9.00 - 10.30 Practicals 6 in groups
Examine BFP cells via microscopy and FACS
Knock-In cell validation - DNA Preparation
10.30 - 12.00 Practicals 6 in groups continues
Examine BFP cells via microscopy and FACS
Knock-In cell validation - DNA Preparation
12.00 - 13.00 Lunch in the EMBL Canteen
13.00 - 13.45

Genome-wide and targeted screens using CRISPR/Cas9 system
Balca R. Mardin - BioMed X, Germany

13.45 - 15.00 Practical
Knock-In cell validation – Set up Junction PCR
15.00 - 15.30 Coffee Break
15.30 - 16.30 Optimizing CRISPR ribonucleoprotein components for precision genome editing
Justin Barr - Integrated DNA Technologies, United States of America
17.00 Shuttle downtown
from 19.00 Dinner downtown
Friday 21 September 2018
TimeSpeaker
9.00 - 10.00 CRISPR-Cas9 transgenic mice - from concept to founder
Neil Humphreys – EMBL Rome
10.00 - 11.00 Practical
Knock-In cell validation (1) - Gel electrophoresis of junction PCR
11.00 - 11.30

Coffee Break

11.30 - 12.15 Practical
Knock-In cell validation (2) - Analysis of gel electrophoresis
12.15 - 13.00 Final Project Discussion
13.00 - 14.00 Lunch in the EMBL Canteen
14.00 - 16.00

Summary of results, final discussions, feedback session

16.00 - 16.15

Coffee Break

16.15 - 16.30 Closing remarks & End of the course