Location & dates EMBL Heidelberg, Germany 18 - 20 Apr 2018
Deadlines Application closed

Course Overview

The goal of this course is to teach the fundamentals of NGS library preparation and validation. We will start with an introduction into NGS technology and the library preparation workflow. The main part will be the preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for whole genome sequencing data analysis.

Audience

This course is directed towards biologists, scientists and technicians who want to apply whole genome sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

During this course we will focus on the following aspects:

  • Best practices and tips and tricks for TruSeq DNA PCR-free library preparation
  • Best practices of sequencing library validation
  • Introduction to whole genome sequencing data analysis

Learning Outcomes

After this course you should be able to:

  • Prepare libraries from genomic DNA 
  • Quantify and quality control libraries
  • Understand how whole genome sequencing data analysis is performed

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

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  • Olympus logo

Corporate partners

  • 10x Genomics_120x82
  • Becton Dickinson logo
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Sartorius
  • ThermoFisher logo

Associate partners

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  • Nikon logo
  • Nikon logo
  • Sanofi logo