Location & dates EMBL Heidelberg, Germany 18 - 20 Apr 2018 Register Now
Deadlines Application - 24 Jan 2018 Abstract - 24 Jan 2018
organiser_illumina

Course Overview

The goal of this course is to teach the fundamentals of NGS library preparation and validation using the Illumina TruSeq DNA PCR-free sample preparation kit. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for whole genome sequencing data analysis.

Audience

This course is directed towards biologists, scientists and technicians who want to apply whole genome sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

During this course we will focus on the following aspects:

  • Best practices and tips and tricks for TruSeq DNA PCR-free library preparation
  • Best practices of sequencing library validation
  • Introduction to whole genome sequencing data analysis

Learning Outcomes

After this course you should be able to:

  • Prepare libraries from genomic DNA using the Illumina TruSeq DNA PCR-free sample preparation kit
  • Quantify and quality control libraries
  • Understand how whole genome sequencing data analysis is performed

This course is co-organised with illumina.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

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