Location & dates EMBL Heidelberg, Germany 11 - 15 Jun 2018
Deadlines Application closed
organiser_olympus

Programme

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Day 1 - Monday 11 June 2018
Time
08:45 - 09:00 Arrival & Welcome 
09:00 - 13:00 Basics of optics and microscopy
• Theoretical basics of light and optics
• Types of microscope frames – and types of optics
• Set up bright field microscopes and the appropriate optics for different needs
• Cleaning, centering adjusting and operating a microscope
• Basic set up and use of imaging devices
Hands on session 1
13:00 - 14:00 Lunch at EMBL canteen
14:00 - 15:00 Contrast methods
• Introduction into contrast methods
(phase contrast and differential interference contrast/DIC)
15:00 - 17:30 Contrast methods - set up and alignment
• Set up and use of contrast methods:
phase contrast and differential interference contrast/DIC
Hands on session 2
17:30 - 18:00 Discussing open questions
18:00 - 19:00 Dinner at EMBL canteen
19:00 Bus to ISG Hotel
Day 2 - Tuesday 12 June 2018
Time
09:00 - 12:30 Basics of digital imaging
• Adaptation of digital cameras and their alignment
• Interpretation of image content, histogram and digital filter functions
• Improving digital image contrast 
12:30 - 13:30 Lunch at EMBL canteen
13:30 - 16:00 Fluorescence microscopy I
• Introduction to fluorescence microscopy
• Setting up of microscopes and cameras for fluorescence observation
• The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)
• Cubes, filters and objectives
• Find the appropriate filter combination for a given fluorochrome and application
Hands on session 3
16:00 - 17:30 Discussing open questions
17:30 - 18:30 Dinner at EMBL canteen
18:40 Bus to ISG Hotel
Day 3 - Wednesday 13 June 2018
Time
09:00 - 11:30 Fluorescence microscopy II
• Auto-fluorescence – a source for noise
• Digital imaging for fluorescence microscopy
• Specific needs of digital cameras
• Digital multicolor handling
• Acquisition of multidimensional images (3D, time)
Hands on session 3 (continued)
11:30 - 11:35 Group picture
11:35 - 12:30 Guided tour at the EMBL Advanced light microscopy facility (ALMF)
12:30 - 13:30 Lunch at EMBL canteen
13:30 - 15:00 Introduction to the confocal microscope (Talk)
15:00 - 18:00 Hands on session (Group work)
Different applications will be covered in small groups.
Module 1: Multicolor imaging
Module 2: Photomanipulation techniques – FRAP, FLIP and Photoconversion
Module 3: 3D image collection and enhancement – zooming, resolution, SNR, z-stacks, IMARIS basic
Module 4: Time-lapse imaging, bleaching and viability; Multi-Area Time-Lapses and Stitching
18:00 Bus via ISG Hotel to restaurant
19:00 Dinner in downtown Heidelberg
Day 4 - Thursday 14 June 2018
Time
09:00 - 10:00 FRAP and Photoconversion (Talk)
10:00 - 13:00 Hands on session (Group work)
Work in Modules
13:00 - 14:00 Lunch at EMBL canteen
14:00 - 17:00 Hands on session (Group work)
Work in Modules
17:00 - 18:00 Comparative time-lapse overnight experiment on three different setups
18:00 - 19:00 Dinner at EMBL canteen
19:00 Bus to ISG Hotel
Day 5 - Friday 15 June 2018
Time
09:00 - 09:30 Evaluation and summary of overnight timelapse acquisition 
09:30 - 12:30 Hands on session (Group work)
Work in modules 
12:30 - 13:00 Tips and tricks for data presentation
13:00 - 13:20 Closing remarks
13:20 Bus to main train station