Location & dates EMBL Heidelberg, Germany 9 - 14 Jul 2018
Deadlines Application closed
organiser_leica

Programme

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Day 1 - Monday 9 July 2018
TimeLocation
09:00 - 09:15 Welcome
Courtyard A+B
09:15 - 10:15 Overview of current super-resolution microscopy techniques
Marko Lampe, EMBL Heidelberg
Courtyard A+B
10:15 - 10:30

Coffee break

Foyer Courtyard

10:30 - 11:30

Introduction to Course Microscopes (Leica STED 3X)
Ulf Schwarz, Leica Microsystems

Courtyard A+B

11:30 - 12:30

Introduction to Course Microscopes (Leica SR GSD)
Oliver Schlicker, Leica Microsystems

Courtyard A+B

12:30 - 13:30 Canteen Lunch Canteen
13:30 - 14:00 A quantitative map of Condensins on human mitotic chromatids
Nike Walther

Courtyard A+B

14:00 - 16:30 Introduction to the SR GSD microscope and Single colour 2D SMLM using GSDIM / dSTORM (Group 1+2) Part One
Introduction to the STED microscope and Single colour 2D STED
(Group 3+4) Part One

Microscopy rooms 116 A+B

ALMF 509D + 516B
16:30 - 17:00 Coffee break Foyer Courtyard
17:00 - 19:30

Introduction to the SR GSD microscope and Single colour 2D SMLM using GSDIM / dSTORM (Group 3+4) Part One
Introduction to the STED microscope and Single colour 2D STED
(Group 1+2) Part One

Microscopy rooms 116 A+B

ALMF 509D + 516B
19:30 - 20:30 Canteen Dinner Canteen
20:30 Shuttle bus to ISG Hotel
Day 2 - Tuesday 10 July 2018
Time
09:00 - 09:45

Flash talks by participants

Courtyard A+B

09:45 - 10:00 Coffee break Foyer Courtyard
10:00 - 10:45 Flash talks by participants Courtyard A+B
10:45 - 11:00 Coffee break Foyer Courtyard
11:00 - 12:00 Zooming into Golgi biology with super-resolution imaging
Francesca Bottanelli, Yale School of Medicine
Courtyard A+B
12:00 - 13:00 Canteen Lunch Canteen
13:00 - 14:00 DNA spatial organization by OxEA based Multicolor super resolution Microscopy
Leila Nahidiazar, Hubrecht Institute
Courtyard A+B
14:00 - 15:15 Thunderstorm Tutorial Computer Training Lab
15:15 - 15:30 Coffee break

Foyer Courtyard

15:30 - 17:30 Multicolor 3D GSDIM / dSTORM (Group 1)
PALM imaging (Group 2)
Multicolour 3D STED (Group 3)
Live Cell STED (Group 4)

Microscopy rooms 116 A+B

ALMF 509D + 516B
17:30 - 17:45 Coffee break
Foyer Courtyard
17:45 - 19:45 Multicolor 3D GSDIM/dSTORM (Group 2)
PALM imaging (Group 1)
Multicolour 3D STED (Group 4)
Live Cell STED (Group 3)

Microscopy rooms 116 A+B

ALMF 509D + 516B
19:50 Shuttle bus to ISG Hotel
20:00 Dinner and Worldcup Semi-Final at ISG Hotel
Day 3 - Wednesday 11 July 2018
Time
09:00 - 09:30

Contextual super resolution imaging - fine structure preservation for light- and electron microscopy
Jana Döhner, University of Zurich

Courtyard A+B

09:30 - 09:45 Coffee break Foyer Courtyard
09:45 - 11:15 3D-SMLM, 4pi and adpative optics
Jörg Bewersdorf, Yale University School of Medicine
Courtyard A+B
11:15 - 11:30 Coffee break Foyer Courtyard
11:30 - 12:30 Protein counting at the nano-scale with single-molecule super-resolution imaging
Mike Heilemann, Johann Wolfgang Goethe-University Frankfurt
Courtyard A+B
12:30 - 13:20 Canteen Lunch
Canteen
13:20 - 13:30 Group picture Foyer Courtyard
13:30 - 14:30 Putting things into context: Super-resolution shadow imaging of brain structure
Valentin Nägerl, University of Bordeaux, France

Courtyard A+B

14:30 - 16:30 Multicolour 3D GSDIM / dSTORM (Group 3)
PALM imaging (Group 4)
Multicolour 3D STED (Group 1)
Live Cell STED (Group 2)

Microscopy rooms 116 A+B

ALMF 509D + 516B
16:30 - 17:30 Information in localisation microscopy
Susan Cox, King´s College London
Courtyard A+B
17:30 - 17:45 Coffee break

Foyer Courtyard

17:45 - 19:45 Multicolour 3D GSDIM / dSTORM (Group 4)
PALM imaging (Group 3)
Multicolour 3D STED (Group 2)
Live Cell STED (Group 1)

Microscopy rooms 116 A+B

ALMF 509D + 516B
19:50 Shuttle bus to ISG Hotel
20:00 Dinner and Worldcup Semi-Final at ISG Hotel
Day 4 - Thursday 12 July 2018
Time
09:00 - 10:00

Towards structural cell biology with single molecule localization microscopy
Jonas Ries, EMBL Heidelberg

Courtyard A+B

10:00 - 11:00 Sample Preparation for Super-Resolution Microscopy

Marko Lampe, EMBL Heidelberg

Courtyard A+B
11:00 - 11:15 Coffee break Foyer Courtyard
11:15 - 11:45 Imaging the Glomerular Filtration Barrier using Stimulated Emission Depletion Microscopy
David Unnersjö Jess, KTH Royal Institute of Technology
Courtyard A+B
11:45 - 12:45 Canteen Lunch Canteen
12:45 - 15:00 Analysis of SMLM data (Group 1+2)
SMLM with PAINT (Group 3+4)

Computer Training Lab /

Microscpy rooms 116 A

ALMF 509D
15:00 - 15:15 Coffee break Foyer Courtyard
15:15 - 17:30

Analysis of SMLM data (Group 3+4)
SMLM with PAINT (Group 1+2)

Computer Training Lab /

Microscopy rooms 116 A
ALMF 509D

17:30 Shuttle to downtown Heidelberg via ISG Hotel
for city tour at 18:15 and course dinner at 19:45
Day 5 - Friday 13 July 2018
TimeLocation
09:00 - 10:00 Introduction to superresolution deconvolution
Hans van der Voort
Courtyard A+B
10:00 - 10:15 Coffee break Foyer Courtyard
10:15 - 11:15

Super-resolution STED-FCS measurements of molecular dynamics
and challenges from biomedical research

Christian Eggeling, University of Oxford

Courtyard A+B

11:15 - 11:30 Coffee break Foyer Courtyard
11:30 - 12:30 In vivo STED microscopy
Katrin Willig, MPI of Experimental Medicine
Courtyard A+B
12:30 - 13:30 Canteen Lunch Canteen
13:30 - 14:30 Dissecting the structure and assembly of nuclear pores and mitotic chromosomes by super-resolution microscopy
Jan Ellenberg, EMBL Heidelberg
Courtyard A+B
14:30 - 15:30 Biocompatible probes for STED imaging of cellular structures
Grazvydas Lukinavicius, Max Planck Institute for Biophysical Chemistry
Courtyard A+B
15:30 - 16:00 Coffee break Foyer Courtyard
16:00 - 16:30 STED-FCS introduction to practical Courtyard A+B
16:30 - 18:00 STED-FCS (Group 1)
Live cell STED II (Group 2)
Deconvolution (Group 3+4)

Mic rooms 116 A+B
ALMF 516B /

Computer Training Lab
18:00 - 18:30 Coffee break Foyer Courtyard
18:30 - 20:00 STED-FCS (Group 2)
Live cell STED II (Group 1)
Deconvolution (Group 3+4)

Mic rooms 116 B
ALMF 516B /

Computer Training Lab
20:00 - 21:00 Canteen Dinner
Canteen
21:00 Shuttle bus to ISG Hotel
Day 6 - Saturday 14 July 2018
Time
09:00 - 10:30 STED-FCS (Group 3)
Live cell STED II (Group 4)
Deconvolution (Group 1+2)

Microscopy rooms 116 B
ALMF 516B /

Computer Training Lab
10:30 - 10:45 Coffee break Foyer Courtyard
10:45-12:15 STED-FCS (Group 4)
Live cell STED II (Group 3)
Deconvolution (Group 1+2)

Microscopy rooms 116 B
ALMF 516B /

Computer Training Lab
12:15 - 13:15 Canteen Lunch Canteen
13:15 - 14:00 STED-FCS-data analysis Courtyard A+B
14:00 - 14:30 Planning of Super-Resolution Microscopy projects and troubleshooting for sample preparation Courtyard A+B
14:30 - 14:45 Coffee break Foyer Courtyard
14:45 - 17:45 Imaging of advanced samples for STED and GSDIM with practical tasks for the groups

Microscopy rooms 116 A+B
ALMF 516B / ALMF 509D

Computer Training Lab
17:45 - 18:15 Feedback and wrap-up Courtyard A+B
18:20 Shuttle bus to train station and ISG Hotel