Location & dates EMBL Heidelberg, Germany 20 - 23 Mar 2018
Deadlines Application closed

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Programme

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Programme still subject to change

Day 1 - Tuesday 20 March 2018
TimeSpeakerLocation
13.00 - 14.00

Arrival & Registration

Flex Lab A & B
14.00 - 15.00

Welcome & Introduction

Flex Lab A & B
15.00 - 18.00

Lecture - Best practise: Handling of beads
Get familiar with the NEBNext Ultra II Directional RNA protocol
Questions on protocol 
Keerthana Krishnan - NEB (New England Biolabs)

Flex Lab A & B
18.00 - 19.00

Dinner

EMBL Canteen
19.00

Bus to Hotel

Day 2 - Wednesday 21 March 2018
TimeSpeakerLocation
09.00

Practical - RNA Library Prep Part 1
Keerthana Krishnan - NEB (New England Biolabs)
Dinko Pavlinic - EMBL Heidelberg
Nayara Azevedo - EMBL Heidelberg

Training Lab B
12.00 - 13.00

Lunch

EMBL Canteen
13.00 - 14.00

Pratical - RNA Library Prep Part 2
Keerthana Krishnan - NEB (New England Biolabs)
Dinko Pavlinic - EMBL Heidelberg
Nayara Azevedo - EMBL Heidelberg

Training Lab B
14.00 - 15:00

Lecture - Title TBC
Andreas Burchert - Universitätsklinik Marburg, Germany

 FLex LAb A & B
15.00 - 16:00

Tour of the GeneCore Facilities

16.00 - 17.00

Lecture - Use of NEBNext Ultra II Directional RNA with low quality and FFPE-derived RNA
Keerthana Krishnan - NEB (New England Biolabs)

Flex Lab A & B
17.00 - 18.00

Lecture - Overview about general technical questions for NEBNext library preparation
Keerthana Krishnan - NEB (New England Biolabs)

Flex Lab A & B
18.00 - 19.00

Dinner

EMBL Canteen
19.00 Bus to Hotel

Day 3 - Thursday 22 March 2018
TimeSpeakerLocation
09.00 - 12.00 Practical - RNA Library Prep Part 3
Keerthana Krishnan - NEB (New England Biolabs)
Dinko Pavlinic - EMBL Heidelberg
Nayara Azevedo - EMBL Heidelberg

Flex Lab A & B
12.00 - 13.00 Lunch

EMBL Canteen
13.00 - 18.30

Practical - RNA Library Prep Part 4
Quantification of Libraries and set up of Sequencing run
Keerthana Krishnan - NEB (New England Biolabs)
Dinko Pavlinic - EMBL Heidelberg
Nayara Azevedo - EMBL Heidelberg

Training Lab B
18.30

Bus to Bismarkplatz

19.30

Dinner Downtown

Day 4 - Friday 23 March 2018
TimeSpeakerLocation
09.00 - 10.00

Lecture - Systematic analysis of EnigmRBPs. Pushing the limits of RNA sequencing
Claudio Asencio Salcedo - EMBL Heidelberg

Computer Training Lab
10.00 - 12.30

Practical - Bioinformatics I
Jonathan Landry - EMBL Heidelberg
Jan Provaznik - EMBL Heidelberg

Computer Training Lab
12.30 - 13.30

Lunch

Flex Lab A
13.30 - 15.45

Practical - Bioinformatics II
Jonathan Landry - EMBL Heidelberg
Jan Provaznik - EMBL Heidelberg

Computer Training Lab
15.45 - 16.00 Closing Remarks and Departure

Computer Training Lab