Location & dates EMBL Heidelberg, Germany 2 - 5 Jul 2018
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organiser_roche

Programme

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Day 1 - Monday, 2 July 2018
TimeSpeakerLocation
14:00 - 14:30

Arrival & Welcome

Courtyard Room A+B

14:30 - 15:30

Keynote Speaker
Toumy Guettouche - Roche Sequencing Solutions, USA 

Courtyard Room A+B

15:30 - 16:00

Coffee break

Courtyard Room A+B

16:00 - 16:45

Strategies for the construction of high complexity and low-bias NGS libraries
Frank Unterseher, Roche Diagnostics, Germany 

Courtyard Room A+B

16:45 - 17:30

Library preparation strategies for RNA-Seq
Rafael Szczepanowski, Roche Diagnostics, Germany

Courtyard Room A+B

17:30 - 18:00

Planning hands-on part for days 2 & 3
Frank Unterseher, Roche Diagnostics, Germany
Rafael Szczepanowski, Roche Diagnostics, Germany

Courtyard Room A+B

18:00 - 19:00

BBQ and 2018 FIFA World Cup Russia™, Round of 16 viewing

ISG Hotel

Day 2 - Tuesday, 3 July 2018
TimeSpeakerLocation
09:00 - 10:00

Review of RNA quality and quantity, concentration adjustment

Training Lab A

10:00 - 10:30

RiboErase reaction

Training Lab A

10:30 - 11:15

Depletion

Training Lab A

11:15 - 11:45

Clean up

Training Lab A

11:45 - 12:15

DNase digest

Training Lab A

12:15 - 13:15

Lunch break

EMBL Canteen

13:15 - 13:45

Clean up

Training Lab A
13:45 - 14:45

Elution – Fragmentation – Priming

Training Lab A

14:45 - 15:00

Fragmentation – Priming

Training Lab A

15:00 - 15:50

1st strand synthesis

Training Lab A

15:50 - 16:35

2nd strand synthesis

Training Lab A

16:35 - 16:55

Adapter ligation

Training Lab A

16:55 - 17:25

Clean up 1

Training Lab A

17:25 - 17:55

Clean up 2

Training Lab A
17:55 - 18:00

Amplification

Training Lab A
18:00 - 19:00 Dinner at Restaurant Delphi
Day 3 - Wednesday, 4 July 2018
TimeSpeakerLocation
09:00 - 10:00

Sample QC and adjustment

Training Lab A

10:00 - 10:45

Fragmentation (2 times / person)

Training Lab A

10:45 - 11:20

End Repair & A-tailing

Training Lab A

11:20 - 12:00

Adapter Ligation

Training Lab A

12:00 - 12:30

Clean up

Training Lab A

12:30 - 13:30

Lunch break

EMBL Canteen
13:30 - 14:00

Double Sided Size Selection

Training Lab A

14:00 - 14:30

Amplification

Training Lab A

14:30 - 15:00

Clean up

Training Lab A

15:00 - 15:30

KAPA Library Quant kit overview
Rafael Szczepanowski, Roche Diagnostics, Germany 

Courtyard Room A+B

15:30 - 16:30 QC of RNA and DNA libraries (NanoDrop, Qubit, Bioanalyzer) Courtyard Room A+B
16:30 - 17:00 Coffee break Courtyard Room A+B
17:00 - 18:00 Preparation of qPCR Training Lab A
18:00 Start of qPCR Training Lab A
18:00 Bus to ISG Hotel, depart of guided tour at 18:30
19:00 - 21:00 Guided Tour of Heidelberg & Course dinner Downtown Heidelberg
Day 4 - Thursday, 5 July 2018
TimeSpeakerLocation
09:00 - 10:00

Systematic analysis of EnigmRBPs. Pushing the limits of RNA sequencing
Claudio Asencio Salcedo, EMBL Heidelberg, Germany

Computer Training Lab

10:00 - 12:30

Bioinformatics I
Jonathan Landry and Jan Provaznik, EMBL Heidelberg, Germany

Computer Training Lab

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Analysis of qPCR Results

Computer Training Lab

14:30 - 16:45

Bioinformatics II
Jonathan Landry and Jan Provaznik, EMBL Heidelberg, Germany

Computer Training Lab

16:45 - 17:00

Closing remarks and departure

Computer Training Lab