Location & dates EMBL Heidelberg, Germany 15 - 18 May 2018 Register Now
Deadlines Application - 27 Feb 2018
organiser_BioRad_Illumina
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Programme

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The actual programme is currently being finalised. A daily overview can be found below:

Day 1 - Tuesday 15 May 2018
TimeSpeakerLocation
10:30 - 12:00

Lecture: Introduction to ddSEQ

Flex Lab A & B

12:00 - 13:00 Lunch EMBL Canteen
13:00 - 14:30

Lecture: 3SureCell Troubleshooting & Best Practices

Flex Lab A & B

14:30 - 15:30

Lecture: Illumina Sequencing Overview

Flex Lab A & B

15:30 - 16:00

Coffee break

Flex Lab A & B

16:00 - 17:30 Lecture: NextSeq Workflow & BaseSpace Overview

Flex Lab A & B

17:30 - 18:00 Lecture: Customer Resources

Flex Lab A & B

18:00 - 19:00 Dinner

EMBL Canteen

Day 2 - Wednesday 16 May 2018
TimeSpeakerLocation
08:15 - 10:15

Practical: Cell Prep

Training Lab B

10:15 - 10:30

Coffee break

Flex Lab A & B

10:30 - 11:00

Practical: Cell Buffer Prep

Training Lab B

11:00 - 11:30

Practical: Droplet Generation (ddSEQ)

Training Lab B

11:30 - 12:15

Practical: Set up RT (1st Strand Synthesis)

Training Lab B

12:15 - 13:30

Lunch

EMBL Canteen

13:30 - 14:15

Practical: Emulsion Breakage

Training Lab B
14:15 - 15:30

Practical: Clean Up 1st Strand Synthesis

Training Lab B

15:30 - 16:30

Practical: Set up 2nd Strand Synthesis

Training Lab B

16:30 - 17:00

Coffee break

Flex Lab A & B

17:00 - 18:00 Lecture: Technical and biological considerations using single-cell gene expression profiling: from tissue to data analysis
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Flex Lab A & B

18:00 - 19:00 Dinner EMBL Canteen
Day 3 - Thursday 17 May 2018
TimeSpeakerLocation
08:15 - 09:00

Practical: Clean up 2nd Strand Synthesis

Training Lab B

09:00 - 09:30

Practical: Set up Bioanalyzer

Training Lab B

09:30 - 10:30 Gene Core Tour

10:30 - 10:45

Coffee Break

Flex Lab A & B

10:45 - 11:15

Practical: Tagmentation

Training Lab B

11:15 - 12:15

Practical: Set up PCR

Training Lab B

12:15 - 13:15

Lunch

EMBL Canteen

13:15 - 14:15

Practical: Clean up PCR

Training Lab B

14:15 - 14:30

Practical: Set up Bioanalyzer

Training Lab B

14:30 - 15:15

Coffee Break

Flex Lab A & B

15:15 - 15:45

Practical: Prepare the NextSeq500 Reagents

Training Lab B

15:45 - 16:15

Practical: Run Set Up in PrepTab

Training Lab B

16:15 - 16:45

Practical: Normalize and Pool

Training Lab B

16:45 - 18:00 Practical: NextSeq Run start Training Lab B
18:00 - 19:00 Downtonw dinner
Day 4 - Friday 18 May 2018
TimeSpeakerLocation
08:15 - 08:45

Lecture: Basespace App start

Flex Lab A & B

08:45 - 09:00 Coffee break

Flex Lab A & B

09:00 - 12:00

Lecture: Data Analysis

Flex Lab A & B

12:00 - 13:00

Lunch Break

EMBL Canteen

13:00 - 14:30

Lecture: Introduction to BaseSpace and Secondary Analysis for Single-Cell RNA-Seq

Flex Lab A & B

14:30 - 15:00

Coffee Break

Flex Lab A & B

15:00 - 16:30

Lecture: Introduction to Tertiary Analysis for Sinlge-Cell RNA-Seq

Flex Lab A & B

16:30

Departure