EMBL Courses and Conferences during the Coronavirus pandemic
With the onsite programme paused, many of our events are now being offered in virtual formats.
Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.
More information for participants of events at EMBL Heidelberg can be found here.
Course Overview
This course will provide participants with hands-on tutorials and the theory behind gene transfer into fish embryo, as well as gene knock-in/-out using CRISPR/Cas. Microinjection into zebrafish and medaka embryos will be covered, as well as subsequent screening to identify positive founder fish.
Audience
This course is aimed at research technicians and scientists interested in the generation of genetic modified fish models. Participants should have basic experience with molecular biology techniques.
Modules/Resources
- Microinjection of lineage tracers into one-cell stage embryos
- Co-injection of DNA and mRNA into one-cell stage embryos
- Microinjection of CRISPR/Cas constructs
- Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
- Homology directed repair in fish
- Screening by imaging
- High throughput, high content screening for successful events
- Molecular validation of Crispr/Cas9 mediated indel formation
Learning Outcomes
The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.
