This course on the fundamentals of high-end cell sorting will enable scientists to properly use the global upstream technology of cell sorting with high confidence. Cell sorting is essential to many studies, but researchers outside of core facilities have very limited knowledge how exactly sorting works and unknowingly produce erroneous samples or even scientifically crippling data.
The course is intended for PhD students and postdocs who are starting to use, or already using flow cytometry based cell sorting in their research projects. Applicants with some previous active knowledge of basic principles of flow cytometry and a regular use of flow cytometers in daily research will profit most from the content of the course.
The topics covered will be the common principles of cell sorting applicable to all droplet sorters, how to properly maintain and evaluate the principle components of a cell sorter and how to correct or avoid technical insufficiency, performing single cell sorts for sequencing or any other common down-stream method. The course will provide knowledge transfer from EMBLs Gene Core with regards to single cell genomics. Our main aim is to provide our attendees with the right knowledge and experience about cell sorting so that they can go back to their research with high confidence about their sorts.
The principle objectives are to deliver the theory of cell sorting as well as the practical sides of this high-end technology.
This course is kindly supported by The Flow Cytometry Core Facility at EMBL Heidelberg.