The aim of this course is to prepare and analyse "bisulfite-free" methylome libraries for Illumina sequencing. We will use a novel enzymatic approach to detect 5-methyl cytosines (5mC's), that results in longer reads and higher coverage of genomic features at lower minimum coverage thresholds when compared to Illumina bisulfite libraries.
This course is directed towards wet-lab biologists, scientists and technicians who want to apply the bisulfite-free method for detecting 5mC's to their samples. Participants will learn the best practice of library preparation and data analysis. Knowledge of next generation sequencing technology is desirable but not essential.
- Enzymatic detection of 5mC's
- Introduction to methylome data analysis
Preparation of methylome libraries using an enzymatic approach. Introduction to data analysis of these libraries.