Location & dates EMBL Heidelberg, Germany 2 - 5 Dec 2019 Register Now
Deadlines Application - 9 Sep 2019
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Course Overview

The aim of this course is to prepare and analyse "bisulfite-free" methylome libraries for Illumina sequencing. We will use a novel enzymatic approach to detect 5-methyl cytosines (5mC's), that results in longer reads and higher coverage of genomic features at lower minimum coverage thresholds when compared to Illumina bisulfite libraries.

Audience

This course is directed towards wet-lab biologists, scientists and technicians who want to apply the bisulfite-free method for detecting 5mC's to their samples. Participants will learn the best practice of library preparation and data analysis. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

  • Enzymatic detection of 5mC's
  • NGS
  • Introduction to methylome data analysis

Learning Outcomes

Preparation of methylome libraries using an enzymatic approach. Introduction to data analysis of these libraries.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

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  • Olympus logo

Corporate partners

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  • Becton Dickinson logo
  • Bio-Rad
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Milteniy Biotec_logo120
  • Sartorius
  • ThermoFisher logo

Associate partners

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  • Nikon logo
  • Promega logo
  • Roche logo
  • Sanofi logo