Course Overview
Genomic and proteomic approaches have enabled breakthrough advances in the understanding of chromatin biology. This course attempts to integrate these omics approaches to understand the principle of chromatin operation and its biological relevance. Using a murine embryonic stem cells as a tractable model system, this course aims to analyse the composition and variation of chromatin components using antibody and small molecule tools, and the “epigenetic landscape” at key time points following the induction of cellular differentiation.
During the 5 days of the course we will teach how murine stem cells can be differentiated into neuronal progenitors, how specific antibodies and small molecule drugs can be used to enrich for chromatin loci by ChIP- and Chem-seq approaches and how Mass Spectrometry and Next Generation Sequencing read-outs can be used to identify proteins and DNA associated with transcriptionally active chromatin. Combined with the experimental part, a series of lectures given by academic and industry experts in the field will expand the topic of chromatin players and the use of small molecule probes to understand chromatin biology.
Audience
This course is aimed at PhD students and post-doctoral researchers who seek to assess chromatin reorganization and/or are working in the field of neuronal development.
Modules/Resources
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Culture and neuronal differentiation of murine stem cells
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Chromatin sample preparation for genomic and proteomic analysis by means of antibodies and small molecules
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Meta-analysis of post sequencing data
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Integration of MS proteomic data
Learning Outcomes
After this course participants should be able to:
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Cultivate and differentiate neuronal murine stem cells
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Prepare chromatin samples for genomic and proteomic analysis
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Analysis genomic and proteomic data
