Location & dates EMBL Heidelberg, Germany 2 - 6 Sep 2019
Deadlines Application closed
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EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Monday 2 September 2019
TimeSpeakerLocation

Introduction to basics & pull-down

08:40

Bus from ISG Hotel to EMBL

Bus stop in front of ISG Hotel

08:45 - 09:00

Arrival & Registration

Flex Labs A&B

09:00 - 10:00

Welcome, Introduction & Breaking the ice
Paola Grandi - Cellzome/GSK, Germany
Kyung-Min Noh - EMBL Heidelberg, Germany

Flex Labs A&B

10:00 - 12:30

Practical:
Overview experiments

Paola Grandi - Cellzome/GSK, Germany
Kyung-Min Noh - EMBL Heidelberg, Germany

ESC differentiation
Kyung-Min Noh - EMBL Heidelberg, Germany

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 15:00

Practical:
Laboratory work-Cell lysate preparation; Native and cross-link condition

Training Lab B

15:00 - 16:00

Chromatin assembly, conformation, dynamics and accessibility
Karsten Rippe - German Cancer Research Cente (DKFZ) and the Bioquant Center in Heidelberg, Germany

Flex Labs A&B

16:00 - 16:30

Coffee break and meet the speaker

Flex Labs A&B

16:30 - 19:00

Practical (cont.):
Laboratory work-Cell lysate preparation; Micrococcal nuclease and sonication, check digestion pattern

Training Lab B

19:00 - 20:00

Dinner

EMBL Canteen

20:00 - 22:00

Flash Talk Session

ATC Rooftop Lounge

22:00

Bus to ISG Hotel

In front of ATC entrance

Day 2 - Tuesday 3 September 2019
TimeSpeakerLocation

Chem-MS and Chem-seq

08:40

Bus from ISG Hotel to EMBL

Bus stop in front of ISG Hotel

09:00 - 10:00

Identification of clinical candidates among epigenetics targets
Emmanuel Demont - GSK, United Kingdom

Flex Labs A&B

10:00 - 10:30

Coffee break and meet the speaker

Flex Labs A&B

10:30 - 12:30

Practical:
Chem-seq: small-molecule probe preparation. Laboratory work-Chromatin immunoprecipitation for Chem-seq and Mass-spec

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 16:00

Practical:
Preparation of samples for MS; elution from the small molecule-beads, gel running, staining/destaining

Training Lab B

16:00 - 16:30

Coffee break

Flex Labs A&B

16:30 - 17:30

Protein stability proteomics for identification of small molecule targets
Mikhail Savitski - EMBL Heidelberg, Germany

Flex Labs A&B

17:30 - 19:00

Practical (cont.):
Preparation of samples for DNA sequencing

Training Lab B

19:00 - 20:00

Dinner

EMBL Canteen

20:00 - 22:00

Poster Session I

ATC Helix A

22:00

Bus to ISG Hotel

In front of ATC entrance

Day 3 - Wednesday 4 September 2019
TimeSpeakerLocation

ChIP-MS and ChIP-seq

08:40

Bus from ISG Hotel to EMBL

Bus stop in front of ISG Hotel

09:00 - 10:30

Identification of chromatin-associated protein networks
Jeroen Krijgsveld - German Cancer Research Center (DKFZ), Germany

Introduction on MS
Nico Zinn - Cellzome/GSK, Germany

Flex Labs A&B

10:30 - 10:45

Coffee break and meet the speaker

Flex Labs A&B

10:45 - 12:45

Practical:
Chromatin immunoprecipitation for ChIP-seq and Mass-spec; DNA-Protein (mass spec analysis of proteins and in parallel sequencing of the DNA)
Mandy Rettel - EMBL Heidelberg, Germany

Training Lab B

12:45 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Histone PTM proteomics: investigate multi-layered gene regulation
Tiziana Bonaldi - European Institute of Oncology, Italy

Flex Labs A&B

14:30 - 15:30

Practical:
Tandem Mass Tag (TMT) labeling

Training Lab B
from 15:30

Free afternoon / evening

15:35

Bus to ISG Hotel

In front of ATC entrance
16:15

Bus from ISG Hotel to Neckarmünzplatz

Bus stop in front of ISG Hotel
from 16:30

Optional - guided tour of Heidelberg

Neckarmünzplatz
Day 4 - Thursday 5 September 2019
TimeSpeakerLocation

NGS Library generation

08:40

Bus from ISG Hotel to EMBL

Bus stop in front of ISG Hotel

09:00 - 10:00

Using variation across individuals for generating cell-type specific gene regulatory networks
Judith Zaugg- EMBL Heidelberg, Germany

Flex Labs A&B

10:00 - 10:30

Coffee break and meet the speaker

Flex Labs A&B

10:30

Group photo

10:30 - 12:30

Practical:
Library generation, Preparation of sample for MS: gel-cutting, digestion and elution
Mandy Rettel - EMBL Heidelberg, Germany

Training Lab B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 16:00

Practical:
Library generation, Submitting samples for sequencing and protein identification
Mandy Rettel - EMBL Heidelberg, Germany

Training Lab B

16:00 - 16:30

Coffee break

Flex Labs A&B

16:30 - 17:30

Genomic-pipeline
Vladimir Benes - EMBL Heidelberg, Germany

Flex Labs A&B

17:30 - 18:30

Tour of Genecore Facility and Proteomics Facility
Dominic Helm - EMBL Heidelberg, Germany

Genecore Facility
Proteomics Facility

18:30 - 19:00

Q&A Session
Dominic Helm - EMBL Heidelberg, Germany

Flex Labs A&B

19:00 - 20:00

Dinner

EMBL Canteen
20:00 - 21:30

Poster Session II

ATC Helix A
21:30

Bus to ISG Hotel

EMBL public bus stop
Day 5 - Friday 6 September 2019
TimeSpeakerLocation

Data Analysis

08:40

Bus from ISG Hotel to EMBL

Bus stop in front of ISG Hotel

09:00 - 10:00

Protein-chromatin interaction analysis
Charles Girardot - EMBL Heidelberg, Germany

Computer Training Lab

10:00 - 10:30 Coffee break and meet the speaker

Computer Training Lab

10:30 - 12:30

Practical:
GBCS- ChIP-seq data analysis with Galaxy

Charles Girardot - EMBL Heidelberg, Germany

Computer Training Lab

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

The developing mouse nervous system in the light of histone methylation
Tanja Vogel - University of Freiburg, Germany

Computer Training Lab

14:30 - 15:30

Practical:
GSK MS-data analysis
Maria Faelth-Savitski - Cellzome/GSK, Germany

Computer Training Lab
15:30 - 16:00

Coffee break and Q&A Session

Computer Training Lab

16:00 - 17:30

Practical (cont.):
GBCS- ChIP-seq and GSK MS-data analysis
Maria Faelth-Savitski - Cellzome/GSK, Germany
Charles Girardot - EMBL Heidelberg, Germany
Mathias Andres Saver Cortes - EMBL Heidelberg, Germany
Jelle Scholtalbers - EMBL Heidelberg, Germany

Computer Training Lab

17:30 - 18:30

Wrap-up and closing of the course

Computer Training Lab
18:40

Bus to ISG Hotel

In front of ATC entrance
19:10

Bus to Kulturbrauerei

Bus stop in front of ISG Hotel
from 19:30

Course Dinner

Kulturbrauerei Heidelberg