Location & dates EMBL Heidelberg, Germany 17 - 22 Mar 2019
Deadlines Application closed
Organiser_ThermoFisher

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Sunday 17 March 2019
TimeSpeakerLocation
from 17:00

Arrival & Welcome

ISG Hotel

Welcome round of organisers, trainers, speakers

ISG Hotel

Ice-breaker & Flash Talks

18:30 - 19:30

Introduction into CRISPR/Cas9
Sibylle Vonesch - EMBL Heidelberg, Germany

ISG Hotel

19:30

Dinner

ISG Hotel

Day 2 - Monday 18 March 2019
TimeSpeakerLocation
09:00 - 09:30

Welcome to EMBL
Yvonne Yeboah, Elisabeth Zielonka - EMBL Heidelberg, Germany

Flex Lab A&B

09:30 - 10:15

Purification of Cas9 protein
Kim Remans - EMBL Heidelberg, Germany

Flex Lab A&B

10:15 - 10:45

Coffee break

Flex Lab A&B

10:45 - 11:45

gRNA/Donor design (Intro to practical)
Thermo Fischer Scientific

Flex Lab A&B

11:45 - 12:30

Practical 1-1
Guide RNA Synthesis – PCR for IVT templates

Flex Lab A&B
12:30 - 13:30

Lunch break

EMBL Canteen
13:30 - 14:30

The 3Cs technology for CRISPR gRNA library generation
Manuel Kaulich - Goethe University, Germany

Flex Lab A&B
14:30 - 15:00

Coffee break

Flex Lab A&B
15:00 - 16:30

Practical 1-2
PCR gel analysis and set up IVT

Training Lab B
16:30 - 18:00

Practicals 2 & 3 in groups
Transfection of 293FT cells with gRNA & Cas9mRNA lipofection
Electroporation of 293FT with gRNA & Cas9protein
Guide RNA design task

Training Lab B
18:00 - 19:00

Dinner

EMBL Canteen
19:00 - 20:30

Practicals 2 & 3 in groups continues
Transfection of 293FT cells with gRNA & Cas9mRNA lipofection
Electroporation of 293FT with gRNA & Cas9protein
Guide RNA design task

Training Lab B
Day 3 - Tuesday 19 March 2019
TimeSpeakerLocation
09:00 - 09:45

Genome and epigenome editing using CRISPR-Cas system
Claudio Mussolino - Medical Center -University of Freiburg, Germany

Flex Lab A&B

09:45 - 10:00

Coffee break

Flex Lab A&B

10:00 - 11:00

Generation and validation of endogenously tagged cell lines
Birgit Koch - Max Planck Institute for Medical Research, Germany

Flex Lab A&B

11:00 - 12:00

Lecture: Introduction to FACS sorting
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A&B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Practicals in groups
a) FACS sorting of knock in cells – single cell sort
b) Individual project discussion

Flex Lab A&B/

Flow Cytometry Core Facility

14:00 - 15:00

Practicals in groups continues
a) Individual project discussion
b) FACS sorting of knock in cells – single cell sort

Flex Lab A&B/

Flow Cytometry Core Facility

15:00 - 15:15

Coffee break

Flex Lab A&B

15:15 - 16:30

Practicals 1-3 & 4 in groups continues
IVT cleanup, quantitation and analysis of gRNA
Transfection of 293FT-eBFP cells using CRISPR/Cas9 for SNP (RNPs)

Training Lab B

16:30 - 16:45

Coffee break

Flex Lab A&B

16:45 - 18:00

Practicals 1-3 & 4 in groups continues
IVT cleanup, quantitation and analysis of gRNA
Transfection of 293FT-eBFP cells using CRISPR/Cas9 for SNP (RNPs)

Training Lab B

18:00 - 20:00

Poster session (odd numbers) and pizza

Top of Helix B

Day 4 - Wednesday 20 March 2019
TimeSpeakerLocation
09:00 - 09:30

High Efficiency Gene Editing using Programmable EnGen Ribonucleoprotein Complexes
Karl von Laer - New England Biolabs, Germany

Flex Lab A&B

09:30 - 10:00

Basic CRISPR in mice
Neil Humphreys  - EMBL Rome, Italy

Flex Lab A&B

10:00 - 10:15

Coffee break

Flex Lab A&B

10:15 - 12:00

Practical 5-1 & 5-2
GCD Cell Harvest and lysis, set up GCD PCR

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 13:45

CRISPR/Cas in yeast
Sibylle Vonesch - EMBL Heidelberg, Germany

Flex Lab A&B
13:45 - 14:15

Practical 5-3
CR analysis, set up GCD re-annealing reaction

Training Lab B
14:15 - 16:15

Practicals 5-4 & 5-5 in groups
Set up digestion for GCD, gel purification & sample submission for sanger sequencing
Gel analysis of GCD

Training Lab B

16:15 - 16:30

Coffee break

Flex Lab A&B

16:30 - 18:30

TIDE - rapid, powerful and easy analysis of CRISPR experiments
Victor Dillard - Desktop Genetics, UK

Flex Lab A&B

18:30 - 21:30

Poster session (even numbers) with tapas buffet

Top of Helix B

Day 5 - Thursday 21 March 2019
TimeSpeakerLocation
09:00 - 10:30

Practicals 6 in groups
Examine BFP cells via microscopy and FACS
Knock-In cell validation - DNA Preparation

Training Lab B

10:30 - 12:00

Practicals 6 in groups continues
Examine BFP cells via microscopy and FACS
Knock-In cell validation - DNA Preparation

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 13:45

Genome-wide and targeted screens using CRISPR/Cas9 system
Balca R. Mardin - BioMed X, Germany

Flex Lab A&B

13:45 - 15:00

Practical
Knock-In cell validation – Set up Junction PCR

Training Lab B

15:00 - 15:30

Coffee break

Flex Lab A&B
15:30 - 16:30

Optimizing CRISPR ribonucleoprotein components for precision genome editing
Justin Barr - Integrated DNA Technologies, United States of America

Flex Lab A&B

17:00

Guided tour of Heidelberg

19:30

Dinner downtown

Day 6 - Friday 22 March 2019
TimeSpeakerLocation
09:00 - 10:00

CRISPR-Cas9 transgenic mice - applications
Neil Humphreys – EMBL Rome

Flex Lab A&B

10:00 - 11:00

Practical
Knock-In cell validation (1) - Gel electrophoresis of junction PCR

Training Lab B

11:00 - 11:30

Coffee break

Flex Lab A&B

11:30 - 12:15

Practical
Knock-In cell validation (2) - Analysis of gel electrophoresis

Training Lab B

12:15 - 13:00

Final Project Discussion

Flex Lab A&B

13:00 - 14:00

Lunch break

EMBL Canteen
14:00 - 16:00

Summary of results, final discussions, feedback session

Flex Lab A&B

16:00 - 16:15

Coffee break

Flex Lab A&B

16:00 - 16:30

Closing remarks & wrap-up

Flex Lab A&B