Arrival and registrations with light lunch

Welcome & introduction
Yvonne Yeboah - EMBL Heidelberg, Germany
Elisabeth Zielonka - EMBL Heidelberg, Germany

Lecture: Humanized mice: from academia to a global market
Leonard Shultz - The Jackson Laboratory, USA

5 min flash talks by speakers
Chair: Renata Stripecke - Hannover Medical School, Germany

5 min flash talks by participants
Chair: Brian Soper - The Jackson Laboratory, USA

Networking and informal dinner downtown

Part 1: Reconstructing the human immune system in vivo
Chair: Renata Stripecke - Hannover Medical School, Germany

Lecture: HSCs and human hematopoiesis in vivo
Dominique Bonnet - The Francis Crick Institute, UK

Flex Lab A & B

Lecture: New strains, immunity and autoimmunity
Michael Brehm - University of Massachusetts Medical School, USA

Flex Lab A & B

Coffee break

Flex Lab A & B

Lecture: T and NK cell development
James Di Santo - Institut Pasteur, France

Flex Lab A & B

Lecture: Human adaptive T and B immunity
Renata Stripecke - Hannover Medical School, Germany

Flex Lab A & B

Lunch break

EMBL Canteen

Lecture: Tools and optimized workflows for the analysis of human cells from humanized mouse models
David Agorklu - Miltenyi Biotech, Germany

Flex Lab A & B

Lecture: Multicolor flow cytometry
Malte Paulsen - EMBL Heidelberg, Germany

Practical: Selection of human hematopoietic stem cells, T cells, monocytes for humanization, harvest tissues from humanized mice and analyses of immune reconstitutions by FACS
Corinna Klein - Heidelberg Institute for Steam Cell technology and Experimental Medicine, Germany
Malte Paulsen - EMBL Heidelberg, Germany
Sebastian Theobald - Hannover Medical School, Germany

Dinner

EMBL Canteen

Poster Session

top of Helix A

Part 2: Infections with human pathogens
Chair: Christian Muenz - University of Zurich, Switzerland

Lecture: EBV and KSV infections
Christian Muenz - University of Zurich, Switzerland

Flex Lab A & B

Lecture: Adenovirus and Ebola virus infections
Estefania Rodriguez-Burgos - Heinrich Pette Institute Hamburg, Germany

Flex Lab A & B

Coffee break

Flex Lab A & B

Lecture: Liver-immune system and innate immune responses against HBV and HCV
Marcus Dorner - Imperial College London, UK

Flex Lab A & B

Lecture: HIV infections
Scott Kitchen - University of California Los Angeles, USA

Flex Lab A & B

Lunch break

EMBL Canteen

Lecture: Histopathology
Estefania Rodriguez-Burgos - Heinrich Pette Institute Hamburg, Germany

Flex Lab A & B

Lecture: Quantitative PCR
Marcus Dorner - Imperial College London, UK

Flex Lab A & B

Practical: DNA samples obtained from mice with infections
(HIV, AdV, EBV, HCMV, Ebola) analyzed by real-time quantitative PCR for quantification of infections. Pre-fixated tissue sections and cells analyzed by for detection of viral antigens by microscopy and flow cytometry, respectively.
Marcus Dorner - Imperial College London, UK
Estefania Rodriguez-Burgos - Heinrich Pette Institute Hamburg, Germany

Dinner

EMBL Canteen

Poster Session

top of Helix A

Part 3: Patient derived transplants (PDX) for oncology research
Chair: J.J. Schuringa - University of Groningen, The Netherlands

Lecture: Ossicle implants for engraftment of hematopoietic stem cells and leukemia
J.J. Schuringa - University of Groningen, The Netherlands

Flex Lab A & B

Lecture: Genetic heterogeneity of AML and pancreatic cancer
Andreas Trumpp - German Cancer Research Center , Germany

Flex Lab A & B

Coffee break

Flex Lab A & B

Lecture: Minimal Information for Patient-Derived Tumor Xenograft Models
Terrence Meehan - European Bioinformatics Institute, UK

Flex Lab A & B

Lecture: Population-scale studies with PDX for precision medicine for colorectal cancer research
Livio Trusolino - University of Torino Medical School, Italy

Flex Lab A & B

Lunch break

EMBL Canteen

Lecture: In silico genomics analyses
Terrence Meehan - European Bioinformatics Institute, UK
J.J. Schuringa - University of Groningen, The Netherlands
Livio Trusolino - University of Torino Medical School, Italy

Flex Lab A & B

Practical: In silico genomics analyses of mutations and tumor heterogeneity analyses, representative examples
Terrence Meehan - European Bioinformatics Institute, UK
J.J. Schuringa - University of Groningen, The Netherlands
Livio Trusolino - University of Torino Medical School, Italy

Dinner

EMBL Canteen

Selected short talks (4x) and round table discussion: challenges in the field

Flex Lab A & B

Part 4: Immuno-oncology and transplantation
Chair: Brian Soper - The Jackson Laboratory, USA

Lecture: PDX models combined with human immune system
James Keck - The Jackson Laboratory, USA

Flex Lab A & B

Lecture: Immuno-oncology of breast cancer
Anja Wege - University Medical Center Regensburg, Germany

Flex Lab A & B

Coffee break

Flex Lab A & B

Lecture: Application of PDX for triple negative marker breast cancer research
Carol Bult - The Jackson Laboratory, USA

Flex Lab A & B

Lecture: Humanized mice and organoids in transplantation research
Kourosh Saeb-Parsy - University of Cambridge, UK

Flex Lab A & B

Lunch break

EMBL Canteen

Lecture:
Krish Mahbubani - University of Cambridge, UK

Flex Lab A & B

Lecture:
Brian Soper - The Jackson Laboratory, USA
Li-Chin Yao - The Jackson Laboratory, USA

Flex Lab A & B

Practical: Humanized mice implanted with patient-derived malignancies
Krish Mahbubani - University of Cambridge, UK
Anja Wege - University Medical Center Regensburg, Germany
Li-Chin Yao - The Jackson Laboratory, USA

Dinner Downtown

Part 5: Regenerative medicine and the regulatory perspective
Karl-Dimiter Bissig - Baylor College of Medicine, USA

Lecture: Development of human liver, iPSCs
Karl-Dimiter Bissig - Baylor College of Medicine, USA

Flex Lab A & B

Lecture: TBC
TBC

Flex Lab A & B

The regulatory perspective of the FDA
Kristina Howard, Food and Drug Administration, USA

Flex Lab A & B

Coffee break

Flex Lab A & B

Lecture: Humanized mice in drug discovery
Johannes Sam - Roche Zurich, Switzerland

Flex Lab A & B

Lecture: Tissue immunophenotyping and cell quantifications
Silvia Guionaud - AstraZeneca, UK

Flex Lab A & B

Lunch break

EMBL Canteen

Round table discussion and closing remarks

Flex Lab A & B