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Correlative Light and Electron Microscopy (CLEM) allows precise localisation of an event of interest within cells and tissues. During this course, we will focus on a specific method that has been developed at EMBL that enables the location of fluorescent proteins with high accuracy at the ultrastructural level. The course will cover both the theory and practicals of high precision CLEM, as well as opportunities to discuss their research with CLEM experts and receive practical and troubleshooting advice.
This course is intended for scientists with experience in EM who want to further develop their skills in high-accuracy correlated microscopy.
The course will combine lectures and hands-on practical work covering all steps in the work flow including cryogenic sample preparation, ultramicroscopy, image registration and electron tomography.
Participants will gain hands-on experience with the CLEM methods and learn tips from the people who developed the methods.