Location & dates EMBL Heidelberg, Germany 23 - 28 Sep 2019
Deadlines Application closed

Programme

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Day 1 - Monday 23 September 2019
TimeSpeakerLocation
08:35

Bus to EMBL

Bus stop in front of ISG Hotel

08:45 - 09:00

Registration

Flex Labs A & B

09:00 - 09:45

Welcome & Introduction

Flex Labs A & B

09:45 - 10:15

Background Lecture
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Flex Labs A & B

10:15 - 10:30

Icebreaker

Flex Labs A & B

10:30 - 10:45

Flashtalks

Flex Labs A & B

10:45 - 11:00

Coffee break

Flex Labs A & B

11:00 - 12:00

Flow Cytometry
Beata Ramasz - EMBL Heidelberg, Germany

Flex Labs A & B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 13:30 SiMSen-Seq workflow
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Flex Labs A & B

13:30 - 15:00

Practical:
DNA extraction from Plasma

Training Lab B

15:00 - 15:15

Coffee break

Flex Labs A & B

15:15 - 16:30

Practical:
Library Preparation

Training Lab B

16:30 - 18:30

Practical:
Clean-Up and quality control

Training Lab B

18:30 - 19:15

Dinner

EMBL Canteen

19:15 - 21:00

Poster session I

ATC Helix A

21:00

Bus to ISG Hotel

EMBL public bus stop

Day 2 - Tuesday 24 September 2019
TimeSpeakerLocation
08:35

Bus to EMBL

Bus stop in front of ISG Hotel

09:00 - 09:15

Outline

Flex Labs A & B

09:15 - 10:30

Practical:
Single Cell Sorting
Beata Ramasz - EMBL Heidelberg, Germany

Training Lab B

10:30 - 10:45

Coffee break

Flex Labs A & B

10:45 - 12:00

Practical:
SiMSen-Seq QC
Library pooling
Start of miSeq

Training Lab B

12:00 - 13:00

Technology lunch

ATC Foyer

13:00 - 13:30

Practical:
Single-cell RT
Bianka Baying - EMBL Heidelberg, Germany
Laura Villacorta - EMBL Heidelberg, Germany

Training Lab B

13:30 - 14:30

Introduction to ddPCR
Francisco Bizouarn - BioRad, USA

Flex Labs A & B
14:30 - 15:00

Practical:
Single-cell qPCR
Bianka Baying - EMBL Heidelberg, Germany
Laura Villacorta - EMBL Heidelberg, Germany

Training Lab B

15:00 - 15:15

Coffee break

Flex Labs A & B
15:15 - 17:00

Practical:
ddPCR

Jörg Bantin - BioRad, USA
Francisco Bizouarn - BioRad, USA

Training Lab B
17:00 - 18:00

Practical:
Introduction into QuantaSoft

Start reading the experiment
Jörg Bantin - BioRad, USA
Francisco Bizouarn - BioRad, USA

Training Lab B

18:00 - 19:00

Dinner

EMBL Canteen

19:00 - 21:00

Poster session II

ATC Helix A

21:00

Bus to ISG Hotel

EMBL public bus stop

Day 3 - Wednesday 25 September 2019
TimeSpeakerLocation
08:35

Bus to EMBL

Bus stop in front of ISG Hotel

09:00 - 09:15

Outline

Flex Labs A & B

09:15 - 10:15

Practical:
Data analysis with QuantaSoft
Jörg Bantin - BioRad, USA
Francisco Bizouarn - BioRad, USA

Training Lab B

10:15 - 10:30

Coffee break

Flex Labs A & B

10:30 - 11:30

Practical:
Data analysis with QuantaSoft
Jörg Bantin - BioRad, USA
Francisco Bizouarn - BioRad, USA

Training Lab B

11:30 - 12:30

Role of biobanks in securing process quality in sample retrieval and storage
Romy Kirsten - National Center for Tumor Diseases (NCT) Heidelberg, Germany

Flex Labs A & B

12:30 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Effect of pre-analytical factors on the measurement of Evs in Liquid Biopsies
An Hendrix - Ghent University, Belgium

Flex Labs A & B

14:30 - 15:30

Clinical validation of droplet-based procedures in the context of liquid biopsies
Valerie Taly - Paris Descartes University, France

Flex Labs A & B
15:30 - 16:00

Coffee break

Flex Labs A & B

16:00 - 17:00

Single-Cell Analysis
Technologies and Applications
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Flex Labs A & B

17:00 - 18:00

Practical:
Genomic analysis of CTC

Training Lab B

from 18:00

Free evening

18:00

Bus to ISG Hotel

EMBL public bus stop
18:15 Bus to Neckarmünzplatz Bus stop in front of ISG Hotel
from 18:30

Optional - guided tour of Heidelberg

Neckarmünzplatz
Day 4 - Thursday 26 September 2019
TimeSpeakerLocation
08:40

Bus to EMBL

Bus stop in front of ISG Hotel

09:00 - 09:15

Outline

Flex Labs A & B

09:15 - 10:30

Exosomal Biomarkers in Liquid Biopsies: How to identify valid and better ‘Biomarker Signatures’ from micro-vesicular small-RNA Sequencing
Michael Pfaffl - Technical University of Munich, Germany

Flex Labs A & B

10:30

Group photo

10:30 - 10:45 Coffee break

Flex Labs A & B

10:45 - 11:00 Workflow overview:
Protein detection
Bianka Baying - EMBL Heidelberg, Germany
Lei Chen - Uppsala University, Sweden
Laura Villacorta - EMBL Heidelberg, Germany

Flex Labs A & B

11:00 - 12:00 Practical:
Plasma Protein detection (PEA)
Detection Probe Set-up
Lei Chen - Uppsala University, Sweden

Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:30

Practical:
Plasma Protein detection (PEA)
qPCR Set-up
Lei Chen - Uppsala University, Sweden

Training Lab B
14:30 - 15:45

Protein detection in Liquid Biopsies
Ulf Landegren - Uppsala University, Sweden

Flex Labs A & B
15:45 - 18:00

Practical:
Plasma Protein detection (PEA)
Demo: Data analysis
Lei Chen - Uppsala University, Sweden

Training Lab B

18:00 Bus to ISG Hotel

EMBL public bus stop

18:40 Bus to restaurant "Wirtshaus zum Nepomuk" Bus stop in front of ISG Hotel
from 19:00

Course dinner

Wirtshaus zum Nepomuk

Day 5 - Friday 27 September 2019
TimeSpeakerLocation
08:40

Bus to EMBL

Bus stop in front of ISG Hotel

09:00 - 09:15

Outline

Computer Training Lab

09:15 - 10:30

Liquid Biopsies in the clinical context
High-fidelity sequencing
Tony E. Godfrey - Boston University School of Medicine, USA

Computer Training Lab

10:30 - 10:45

Coffee break

Computer Training Lab

10:45 - 11:45

CTC, Single-Cell, Two-Tailed PCR for microRNA and cfDNA
Mikael Kubista - Institute of Biotechnology and TATAA Biocenter, Czech Republic

Computer Training Lab

11:45 - 12:45

Lunch break

EMBL Canteen

12:45 - 14:30

Practical:
Single-cell analysis
Data analysis

Computer Training Lab

14:30 - 15:00

Q&A Session

Computer Training Lab
15:00 - 15:30

Coffee break

Computer Training Lab

15:30 - 16:30

Liquid Biopsy: From discovery of metastasis biology to clinical application
Klaus Pantel - University Medical Center Hamburg-Eppendorf, Germany

Computer Training Lab

16:30 - 18:00

Implementing liquid biopsies in routine clinic
What's the need?
Anders Edsjö - Skåne University Hospital, Sweden

Computer Training Lab

18:00

Bus to ISG Hotel

In front of ATC entrance

from 18:30

Flammkuchen Dinner

ISG Hotel

Day 6 - Saturday 28 September 2019
TimeSpeakerLocation
08:40

Bus to EMBL

Bus stop in front of ISG Hotel

09:00 - 09:15

Outline

Computer Training Lab

09:15 - 10:15

Practical:
Single-cell analysis
Data analysis
Mikael Kubista - Institute of Biotechnology and TATAA Biocenter, Czech Republic

Computer Training Lab

10:15 - 10:45 Coffee break

Computer Training Lab

10:45 - 12:15 Practical:
SiMSen-Seq
Data analysis
Gustav Johansson - Sahlgrenska Academy at University of Gothenburg, Sweden
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Computer Training Lab

12:15 - 13:15

Lunch break

EMBL Canteen

13:15 - 14:30

Practical:
SiMSen-Seq
Data analysis
Gustav Johansson - Sahlgrenska Academy at University of Gothenburg, Sweden
Anders Ståhlberg - Sahlgrenska Academy at University of Gothenburg, Sweden

Computer Training Lab

14:30 - 15:00

Wrap up

Computer Training Lab

15:00 - 15:15

End of course

15:15

Bus to ISG Hotel, Heidelberg main train station & to Lufthansa Shuttle bus

EMBL public bus stop