Location & dates EMBL Heidelberg, Germany 13 - 17 May 2019
Deadlines Application closed
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EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Monday 13 May 2019
TimeLocation
08:40 - 09:00 Arrival & Welcome  Courtyard A+B
09:00 - 13:00 Basics of optics and microscopy
• Theoretical basics of light and optics
• Types of microscope frames – and types of optics
• Set up bright field microscopes
and the appropriate optics for different needs

• Cleaning, centering adjusting and operating a microscope
• Basic set up and use of imaging devices
Hands on session 1
Courtyard A+B
13:00 - 14:00 Lunch
EMBL canteen
14:00 - 15:00 Contrast methods
• Introduction into contrast methods
(phase contrast and differential interference contrast/DIC)
Courtyard A+B
15:00 - 17:30 Contrast methods - set up and alignment
• Set up and use of contrast methods:
phase contrast and differential interference contrast/DIC
Hands on session 2
Courtyard A+B
17:30 - 18:00 Discussing open questions Courtyard A+B
18:00 - 19:00 Dinner EMBL cafeteria
19:00 EMBL Bus to ISG Hotel ATC main entrance
Day 2 - Tuesday 14 May 2019
TimeLocation
09:00 - 12:30 Basics of digital imaging
• Adaptation of digital cameras and their alignment
• Interpretation of image content, histogram and digital filter functions
• Improving digital image contrast 
Courtyard A+B
12:30 - 13:30 Lunch EMBL canteen
13:30 - 16:00 Fluorescence microscopy I
• Introduction to fluorescence microscopy
• Setting up of microscopes and cameras for fluorescence observation
• The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)
• Cubes, filters and objectives
• Find the appropriate filter combination for a given fluorochrome and application
Hands on session 3
Courtyard A+B
16:00 - 17:30 Discussing open questions Courtyard A+B
17:30 - 18:40 Dinner EMBL canteen
18:40 EMBL Bus to ISG Hotel ATC main entrance
Day 3 - Wednesday 15 May 2019
TimeLocation
09:00 - 11:30 Fluorescence microscopy II
• Auto-fluorescence – a source for noise
• Digital imaging for fluorescence microscopy
• Specific needs of digital cameras
• Digital multicolor handling
• Acquisition of multidimensional images (3D, time)
Hands on session 3 (continued)
Courtyard A+B
11:30 - 11:35 Group picture
11:35 - 12:30 Guided tour at the EMBL Advanced light microscopy facility (ALMF) ALMF
12:30 - 13:30 Lunch EMBL canteen
13:30 - 15:00 Introduction to the confocal microscope (Talk) Courtyard A
15:00 - 18:00 Hands on session (Group work)
Different applications will be covered in small groups.
Module 1: Multicolor imaging
Module 2: Photomanipulation techniques – FRAP, FLIP and Photoconversion
Module 3: 3D image collection and enhancement – zooming, resolution, SNR, z-stacks, IMARIS basic
Module 4: Time-lapse imaging, bleaching and viability; Multi-Area Time-Lapses and Stitching
Microscopy rooms
18:00 EMBL Bus via ISG Hotel to Restaurant Goldener Falke (Hauptstraße 204) ATC main entrance
19:00 Dinner in downtown Heidelberg
Day 4 - Thursday 16 May 2019
TimeLocation
09:00 - 10:00 FRAP and Photoconversion (Talk) Courtyard A
10:00 - 13:00 Hands on session (Group work)
Work in Modules
Microscopy rooms
13:00 - 14:00 Lunch EMBL Canteen
14:00 - 17:00 Hands on session (Group work)
Work in Modules
Microscopy rooms
17:00 - 18:00 Comparative time-lapse overnight experiment
on three different setups
Microscopy rooms
18:00 - 19:10 Dinner EMBL Canteen
19:10 EMBL Bus to ISG Hotel ATC main entrance
Day 5 - Friday 17 May 2019
TimeLocation
09:00 - 09:30 Evaluation and summary
of overnight timelapse acquisition
Courtyard A
09:30 - 12:30 Hands on session (Group work)
Work in modules
Microscopy rooms
12:30 - 13:00 Tips and tricks for data presentation Courtyard A
13:00 - 13:05 Closing remarks Courtyard A
13:05 - 13:15 Packed lunch Foyer Courtyard
13:15 EMBL Bus via ISG Hotel to main train station ATC main entrance