Location & dates EMBL Heidelberg, Germany 13 - 17 May 2019
Deadlines Application closed
organiser_olympus

Programme

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Day 1 - Monday 13 May 2019
TimeLocation
08:40 - 09:00 Arrival & Welcome  Courtyard A+B
09:00 - 13:00 Basics of optics and microscopy
• Theoretical basics of light and optics
• Types of microscope frames – and types of optics
• Set up bright field microscopes
and the appropriate optics for different needs

• Cleaning, centering adjusting and operating a microscope
• Basic set up and use of imaging devices
Hands on session 1
Courtyard A+B
13:00 - 14:00 Lunch
EMBL canteen
14:00 - 15:00 Contrast methods
• Introduction into contrast methods
(phase contrast and differential interference contrast/DIC)
Courtyard A+B
15:00 - 17:30 Contrast methods - set up and alignment
• Set up and use of contrast methods:
phase contrast and differential interference contrast/DIC
Hands on session 2
Courtyard A+B
17:30 - 18:00 Discussing open questions Courtyard A+B
18:00 - 19:00 Dinner EMBL cafeteria
19:00 EMBL Bus to ISG Hotel ATC main entrance
Day 2 - Tuesday 14 May 2019
TimeLocation
09:00 - 12:30 Basics of digital imaging
• Adaptation of digital cameras and their alignment
• Interpretation of image content, histogram and digital filter functions
• Improving digital image contrast 
Courtyard A+B
12:30 - 13:30 Lunch EMBL canteen
13:30 - 16:00 Fluorescence microscopy I
• Introduction to fluorescence microscopy
• Setting up of microscopes and cameras for fluorescence observation
• The different types of illumination sources (e.g. mercury, xenon, metal halide or LED)
• Cubes, filters and objectives
• Find the appropriate filter combination for a given fluorochrome and application
Hands on session 3
Courtyard A+B
16:00 - 17:30 Discussing open questions Courtyard A+B
17:30 - 18:40 Dinner EMBL canteen
18:40 EMBL Bus to ISG Hotel ATC main entrance
Day 3 - Wednesday 15 May 2019
TimeLocation
09:00 - 11:30 Fluorescence microscopy II
• Auto-fluorescence – a source for noise
• Digital imaging for fluorescence microscopy
• Specific needs of digital cameras
• Digital multicolor handling
• Acquisition of multidimensional images (3D, time)
Hands on session 3 (continued)
Courtyard A+B
11:30 - 11:35 Group picture
11:35 - 12:30 Guided tour at the EMBL Advanced light microscopy facility (ALMF) ALMF
12:30 - 13:30 Lunch EMBL canteen
13:30 - 15:00 Introduction to the confocal microscope (Talk) Courtyard A
15:00 - 18:00 Hands on session (Group work)
Different applications will be covered in small groups.
Module 1: Multicolor imaging
Module 2: Photomanipulation techniques – FRAP, FLIP and Photoconversion
Module 3: 3D image collection and enhancement – zooming, resolution, SNR, z-stacks, IMARIS basic
Module 4: Time-lapse imaging, bleaching and viability; Multi-Area Time-Lapses and Stitching
Microscopy rooms
18:00 EMBL Bus via ISG Hotel to Restaurant Goldener Falke (Hauptstraße 204) ATC main entrance
19:00 Dinner in downtown Heidelberg
Day 4 - Thursday 16 May 2019
TimeLocation
09:00 - 10:00 FRAP and Photoconversion (Talk) Courtyard A
10:00 - 13:00 Hands on session (Group work)
Work in Modules
Microscopy rooms
13:00 - 14:00 Lunch EMBL Canteen
14:00 - 17:00 Hands on session (Group work)
Work in Modules
Microscopy rooms
17:00 - 18:00 Comparative time-lapse overnight experiment
on three different setups
Microscopy rooms
18:00 - 19:10 Dinner EMBL Canteen
19:10 EMBL Bus to ISG Hotel ATC main entrance
Day 5 - Friday 17 May 2019
TimeLocation
09:00 - 09:30 Evaluation and summary
of overnight timelapse acquisition
Courtyard A
09:30 - 12:30 Hands on session (Group work)
Work in modules
Microscopy rooms
12:30 - 13:00 Tips and tricks for data presentation Courtyard A
13:00 - 13:05 Closing remarks Courtyard A
13:05 - 13:15 Packed lunch Foyer Courtyard
13:15 EMBL Bus via ISG Hotel to main train station ATC main entrance