Location & dates EMBL Heidelberg, Germany 14 - 18 Jan 2019
Deadlines Application closed
organiser_Nanopore

EMBL Courses and Conferences during the Coronavirus pandemic

With the onsite programme paused, many of our events are now being offered in virtual formats.


Registration is open as usual for many events, with back-up plans in place to move further courses and conferences online as necessary. Registration fees for any events affected by the COVID-19 disruption are fully refundable.


More information for participants of events at EMBL Heidelberg can be found here.

Programme

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Day 1 - Monday 14 January 2019
TimeSpeakerLocation
12:00

Lunch (optional)

EMBL Canteen

12:45 - 13:00

Registration

Flex Labs A & B

13:00 - 14:00

Welcome & Introduction

Flex Labs A & B

14:00 - 15:00

Flash Talk Session

Flex Labs A & B

15:00 - 15:30

Coffee break

Outside Training Lab Area
15:30 - 16:30

Introduction to Nanopore Sequencing
Sample and library prep theory
QC DNA/RNA samples

Flex Labs A & B
Training Lab B
16:30 - 18:00

Introduction to data analysis principles
Differential gene expression
Splice isoforms
Structural Variation

Flex Labs A & B
Training Lab B

18:00 - 19:00

Dinner

EMBL Canteen

Day 2 - Tuesday 15 January 2019
TimeSpeakerLocation
09:45 - 10:00

Introduction to lab set up and protocols
Barcoded cDNA with PCR
Native barcoding with LSK 109

Flex Labs A & B

10:00 - 11:00

Set up barcoded cDNA PCRs

Flex Labs A & B

11:00 - 11:15

Coffee break

Outside Training Lab Area

11:15 - 13:15

Prepare Native Barcoded LSK 109 libraries

Training Lab B

13:15 - 14:15

Lunch break

EMBL Canteen

14:15 - 14:45

Flow cell loading practice

Training Lab B

14:45  - 15:15

Load LSK109 libraries

Computer Training Lab

15:15 - 15:45

Introduction to MinKNOW and assessing a GridION/MinION run

Training Lab B
15:45 - 16:15

Coffee break

Outside Training Lab Area

16:15 - 16:30 Check AWS connectivity

Training Lab B

16:30 - 17:00 Introduction to the Linux command line and practice

Computer Training Lab

17:00 - 18:00 Tour of EMBL's GeneCore facility

GeneCore facility

18:00 - 19:00

Dinner

EMBL Canteen

Day 3 - Wednesday 16 January 2019
TimeSpeakerLocation
09:45 - 10:15 Review runs from Day 2

Flex Labs A & B

10:15 - 12:00

Finish PCR barcoded libraries

Training Lab B

12:00 - 12:15

Coffee break

Outside Training Lab Area

12:15 - 12:45

Load PCR barcoded libraries

Training Lab B

12:45 - 13:30

Lunch break

EMBL Canteen

13:30 - 14:30

Measuring transcriptional changes in SCraMbLEd yeast genomes with Nanopore-based RNA sequencing
Aaron Brooks - EMBL Heidelberg, Germany

Flex Labs A & B

14:30 - 15:15

Introduction to structural variation and analysis

Computer Training Lab
15:15 - 15:30

Coffee break

Outside Training Lab Area

15:30 - 17:00

Practice structural variation detection with example data

Computer Training Lab

from 17:00

Course Dinner

Restaurant in Downtown Heidelberg

Day 4 - Thursday 17 January 2019
TimeSpeakerLocation
09:45 - 10:15 Review runs from Day 3

Flex Labs A & B

10:15 - 12:15 Structural variation detection with data from Day 2

Training Lab B
Computer Training Lab

12:15 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Nanopore RNA-seq leverages large animal models for systems cardiology research
Christoph Dieterich - Heidelberg University Hospital, Germany

Flex Labs A & B

14:00 - 14:45

Introduction to differential gene expression analysis and alternative splicing

Training Lab B
Computer Training Lab
14:45 - 15:00

Coffee break

Outside Training Lab Area

15:00 - 17:00

Practice differential gene expression and splicing analysis with example data

Training Lab B
Computer Training Lab

from 17:00

Free evening

Day 5 - Friday 18 January 2019
TimeSpeakerLocation
09:45 - 12:15

Differential gene expression and splicing analysis with data from Day 3

Training Lab B
Computer Training Lab

12:15 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:30

Group presentations with data analysis results

Flex Labs A & B

from 14:30

End of course