Location & dates EMBL Heidelberg, Germany 1 - 4 Jul 2019
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organiser_roche

Programme

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Day 1 - Monday 1 July 2019
TimeSpeakerLocation
14:00 - 14:30

Welcome and Introduction

Courtyard Room A+B

14:30 - 15:30

Keynote Lecture: A brief history of sequencing and genomics
Toumy Guettouche - Roche Sequencing Solutions, USA

Courtyard Room A+B

15:30 - 16:00

Coffee Break

Courtyard Room A+B

16:00 - 16:30

Lecture: Strategies for the construction of high complexity and low-bias NGS libraries
Valentina Kovaleva - Roche Diagnostics, Germany

Courtyard Room A+B

16:30 - 17:15

Lecture: Library preparation strategies for RNA-Seq
Carmen Rothmund - Roche Diagnostics, Germany

Courtyard Room A+B

17:15 - 18:20

Flash talks and planning hands-on part for days 2 & 3

Courtyard Room A+B

18:20

Bus to ISG Hotel

ATC Entrance

18:30

BBQ Dinner

ISG Hotel

Day 2 - Tuesday 2 July 2019 (KAPA mRNA HyperPrep Kit)
TimeSpeakerLocation
09:00 - 10:00

Review of RNA quality and quantity, concentration adjustment

Training Lab B

10:00 - 11:40

mRNA capture

Training Lab B

11:40 - 12:20

mRNA elution, fragmentation and priming

Training Lab B

12:20 - 12:30

1st strand synthesis

Training Lab B

12:30 - 13:15

Lunch Break

EMBL Canteen

13:15 - 14:05

2nd strand synthesis

Training Lab B
14:05 - 14:40

Adapter ligation

Training Lab B

14:40 - 15:25

1st post-ligation cleanup

Training Lab B

15:25 - 16:10

2nd post-ligation cleanup

Training Lab B

16:10 - 17:00

Library amplification

Training Lab B

17:00 - 18:00

Library amplification cleanup

Training Lab B

18:00 - 19:00 Pizza Dinner
ATC Foyer
Day 2 - Tuesday 2 July 2019 (KAPA RNA HyperPrep Kit with RiboErase)
TimeSpeakerLocation
09:00 - 09:45

Review of RNA quality and quantity, concentration adjustment

Training Lab B

09:45 - 11:00

Oligo Hybridization and rRNA depletion

Training Lab B

11:00 - 11:45

rRNA depletion cleanup

Training Lab B

11:45 - 12:45

DNase digestion

Training Lab B

12:45 - 13:30

DNase digestion cleanup

Training Lab B

13:30 - 14:15

Lunch Break

EMBL Canteen
14:15 - 14:30

RNA fragmentation and priming

Training Lab B

14:30 - 15:20

1st strand synthesis

Training Lab B

15:20 - 16:15

2nd strand synthesis

Training Lab B

16:15 - 16:50

Adapter ligation

Training Lab B

16:50 - 17:20

1st post-ligation cleanup

Training Lab B

17:20 - 18:00

2nd post-ligation cleanup

Training Lab B

18:00 - 19:00 Pizza Dinner
ATC Foyer
Day 3 - Wednesday 3 July 2019 (KAPA HyperPlus Kit)
TimeSpeakerLocation
09:00 - 10:00

Sample QC and adjustment

Training Lab B

10:00 - 10:45

Enzymatic fragmentation

Training Lab B

10:35 - 11:20

End repair and A-tailing

Training Lab B

11:15 - 12:00

Adapter ligation

Training Lab B

12:00 - 12:30

Post-ligation cleanup

Training Lab B

12:30 - 13:30

Lunch Break

EMBL Canteen
13:30 - 14:00

Double-sided size selection

Training Lab B

14:15 - 14:30

Library amplification

Training Lab B

14:30 - 15:00

KAPA Library Quant Kit overview

Training Lab B

15:00 - 15:30 Post-amplification cleanup

Training Lab B

15:30 - 16:30 QC of RNA and DNA libraries (NanoDrop, Qubit, Bioanalyzer) Courtyard Room A+B
16:00 - 17:00 Coffee Break Courtyard Room A+B
16:30 - 18:00 Preparation of qPCR Training Lab B
17:30 Start of qPCR Training Lab B
18:00 Bus to ISG Hotel ATC Entrance
18:30 Depart for downtown (Neckarmünzplatz) ISG Hotel
19:00- 20:00 Free time
20:00 Course Dinner

Restaurant Goldener Falke
(Hauptstraße 204)

Day 4 - Thursday 4 July 2019
TimeSpeakerLocation
09:00

Group Photo

Meet at Courtyard Room A+B

09:00 - 10:00

Lecture: Systematic analysis of EnigmRBPs. Pushing the limits of RNA sequencing
Claudio Asencio - EMBL Heidelberg, Germany

Computer Training Lab

10:00 - 12:30

Bioinformatics I

Computer Training Lab

12:30 - 13:30

Lunch Break

EMBL Canteen

13:30 - 14:30

Analysis of qPCR Results

Computer Training Lab

14:30 - 16:45

Bioinformatics II

Computer Training Lab

16:45 - 17:00

Closing Remarks and Departure

Computer Training Lab