Location & dates EMBL Heidelberg, Germany 12 - 18 May 2019 Register Now
Deadlines Application - 17 Feb 2019
organiser_EMBO_Course_2017

Course Overview

The analysis of single cells advances research in many fields. To properly deal with such minute amounts of sample, each step of the workflow is critical, from sampling to data analysis. Additionally, the integration of data from different approaches is becoming increasingly important to capture the whole picture. This course will cover established techniques from sampling through sequencing, protein analysis and data analysis. Samples will be collected via flow cytometry, gene expression will be analysed by qRTPCR and next generation sequencing, and protein analysis will be performed by proximity extension assays. In addition, alternative methods for the whole workflow will be covered in interactive sessions of lectures and Q&A with key experts in the field. Data collected by the different methods will be analysed during in-depth sessions, which are a strong component of the course.

Audience

This course targets young scientists entering the field of single cell analysis and wanting to learn about the benefits and limitations of the approach by performing a complete single cell analysis workflow.

Modules/Resources

  • Single cell isolation with FACS
  • Single-cell genomics using Strand-Seq
  • Single-cell epigenetics using bisulfite sequencing
  • Microdroplet-based single-cell transcriptomics
  • Single cell NGS data analysis

Learning Outcomes

After finishing the course, attendees will have an overview of available methods in single cell omics and a basic overview of data analysis of single-cell NGS data sets (genomics, epigenetic and transcriptomics). Attendees will also be able to set up some methods in their lab with the knowledge of the detailed protocol and troubleshooting.

For an impression of the course check the 2017 course programme.

 

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

  • Leica logo
  • Olympus logo

Corporate partners

  • 10x Genomics_120x82
  • Becton Dickinson logo
  • Boehringer Ingelheim logo
  • Eppendorf logo
  • GSK logo
  • Milteniy Biotec_logo120
  • Sartorius
  • ThermoFisher logo

Associate partners

  • Merck logo
  • NEB_logo_120
  • Nikon logo
  • Promega logo
  • Roche logo
  • Sanofi logo