Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify many different parameters from heterogeneous samples, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2017, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.
The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry, data acquisition or analysis software (DIVA + FlowJo) is of advantage, but not strictly a must.
- In-depth principles of Flow Cytometry
- Quality assessment and instrument validation
- Analysis of cell cycle profiles in fixed and live cells
- Proliferation and cell division assessment
- Apoptosis assays
- Tissue staining of cell surface and intracellular antigens
- Principles and guidelines for experimental design, especially for multi-colour Flow Cytometry
- Essential of gating and gating controls
- Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
- Guidelines to prepare flow cytometry data for publication
Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.