Location & dates EMBL Heidelberg, Germany 25 - 29 May 2020 Register Now
Deadlines Application - 2 Mar 2020

Programme

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Please note the bolew programme still subject to changes.

Day 1 - Monday 25 May 2020
TimeSpeakerLocation
08:30 - 09:00

Arrival

Flex Lab A

09:00 - 10:00

Welcome & Introductions

Flex Lab A
10:00 - 12:00

Lecture: Fluidics and Optics
Steffen Schmitt- DKFZ Heidelberg, Germany

Flex Lab A
12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Lecture: Fluorescence & PMTs/Detectors
Jochen Barths - Unité Inserm UMR 1110, France
Lecture: Data Generation, Data Display
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A

15:00 - 15:30

Practical Session: DIVA Software introduction

Training Lab B

15:30 - 19:00

Practical Session: Assessing Instrument performance knoweldge interlude:
What is sensitivity How to evaluate performance every day    
Jochen Barths - Unité Inserm UMR 1110, France
Cora Chadick - Amsterdam UMC, location VUmc, The Netherlands
Andrew Fuller - Newcastle University, UK
Diana Ordonez - EMBL Heidelberg, Germany
Malte Paulsen - EMBL Heidelberg, Germany
Beáta Ramasz - EMBL Heidelberg, Germany
Neda Tafrishi - Application Specialist SONY, Europe

Training Lab B

19:00 - 20:00

Dinner

Day 2 - Tuesday 26 May 2020
TimeSpeakerLocation
09:00 - 10:00

Lecture: The Essentials of Gating, FMO and Proper Internal Gating Controls
Beáta Ramasz - EMBL Heidelberg, Germany

Flex Lab A

10:00 - 10:30 Coffee break Flax Lab A & B
10:30 - 12:00

Lecture: Cell Cycle Analysis
Andrew Fuller - Newcastle University, UK

Flex Lab A

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Practical Session:
Group 1: Measuring univariate and bivariate cell cycle samples
Group 2: Data analysis with FlowJo Session


Flex Lab A
Flex Lab B

15:00 - 15:30

Coffee break

Flex Lab A & B

15:00 - 17:30

Practical Session:
Group 1: Data analysis with FlowJo Session
Group 2: Measuring univariate and bivariate cell cycle samples


Flex Lab B
Flex Lab A

17:30 - 18:30 Lecture: Tips and Tricks for Intracellular Stainings and Fixation
Andrew Fuller - Newcastle University, UK

Flex Lab A

18:30 - 19:30

Dinner

Day 3 - Wednesday 27 May 2020
TimeSpeakerLocation
09:00 - 10:00

Practical Session: Data Analysis of Cell Cycle - FlowJo
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab B

10:00 - 10:30

Coffee break

Flex Lab A & B

10:30 - 12:00

Practical Session: Data Analysis of Cell Cycle – FlowJo
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 14:00

Lecture: Assessing Cell Death
Jochen Barths - Unité Inserm UMR 1110, France


Flex Lab A

14:00 - 15:30

Practical Session: Measuring apoptosis
Jochen Barths - Unité Inserm UMR 1110, France
Cora Chadick - Amsterdam UMC, location VUmc, The Netherlands
Andrew Fuller- Newcastle University, UK
Diana Ordonez - EMBL Heidelberg, Germany
Malte Paulsen - EMBL Heidelberg, Germany
Beáta Ramasz - EMBL Heidelberg, Germany
Neda Tafrishi - Application Specialist SONY, Europe

Trainign Lab B

15:30 - 17:00

Practical Session: Data analysis Apoptosis - FlowJo

Flex Lab B
17:00 - 21:00

Social Activity & Downtown Dinner

Day 4 - Thursday 28 May 2020
TimeSpeakerLocation
09:00 - 10:00

Lecture: Fluorescence Spillover and Compensation
Cora Chadick - Amsterdam UMC, location VUmc, The Netherlands

Flex Lab A

10:00 - 11:00

Lecture: Experimental Design for Multi-colour Flow
Diana Ordonez - EMBL Heidelberg, Germany

Flex Lab A

11:00 - 12:00

Lecture: “Expanding capabilities: BDTM AbSeq Technology and the BD RhapsodyTM System to complement Flow”
Wieland Keilholz – BD Biosciences, Germany

Flex Lab A

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 15:00

Practical Session: 
Group 1: Measuring multi-colour surface staining 
Group 2: Data analysis with FlowJo


Training Lab B
Flex Lab B

15:00 - 15:30

Coffee break

15:30 - 17:30

Practical Session:
Group 1:  Data analysis with FlowJo
Group 2:  Measuring multi-colour surface staining


Flex Lab B
Training Lab B

17:30 - 18:30

Lecture: How to Prepare Flow Cytometry Data for Publication

Flex Lab A

18:30

Dinner

Day 5 - Friday 29 May 2020
TimeSpeakerLocation
09:00 - 10:00

Lecture: The Basics of Cell Sorting
Malte Paulsen - EMBL Heidelberg, Germany

Flex Lab A

10:00 - 12:00

Parallel Sessions:
Session N1: Cell sorting  demonstration and reanalysis of sorted cells
Diana Ordonez - EMBL Heidelberg, Germany

Session N2: New tools for multi-parametric analysis in FlowJo
Malte Paulsen - EMBL Heidelberg, Germany

Session N3: Experiment set-up in the cytometer
Beáta Ramasz - EMBL Heidelberg, Germany

FCCF EMBL


Flex Lab B


Training Lab B

12:00 - 13:00

Lunch break

EMBL Canteen

13:00 - 13:30

Feedback and Good-bye

ATC Rooftop Lounge

13:40

Bus to Train Station & Crown Plaza Hotel